FIGURE SUMMARY
Title

Zebrafish Model for Screening Antiatherosclerosis Drugs

Authors
Han, J., Zhang, R., Zhang, X., Dong, J., Chen, M., Pan, Y., Liao, Z., Zhong, M., He, J., Wang, F., Yue, Y., Shang, J.
Source
Full text @ Oxid Med Cell Longev
Figure 1

Early symptoms of AS zebrafish. (a) Cholesterol accumulates in the blood vessels of AS zebrafish. The numbers of macrophages (b) and neutrophils (c) in the blood vessels of AS zebrafish. The mRNA expressions of vcam-1b (d), tnf-α (e), and il-6 (f) of 3 AS zebrafish. Representative images and bar graphs (mean ± SD) were expressed, n=4.

Figure 2

Disturbance of lipid metabolism and oxidative stress in early AS zebrafish. Effects of HCD, LPS, and HCD+LPS on the lipid level (a), TC (b), and TG (c) content of AS zebrafish. Effects of HCD, LPS, and HCD+LPS on the ROS activity (d), MDA content (e), and SOD activity (f) of AS zebrafish. Representative images and bar graphs (mean ± SD) were expressed, n=4.

Figure 3

Plaque formation of AS zebrafish. (a) The results of HE staining showed that plaques were formed in the blood vessels of the 3 AS zebrafish, causing vascular stenosis. (b) The results of EVG staining showed that the plaques of the 3 AS zebrafish all contained fibrous tissue. (c) The results of Oil red O staining showed that the plaques of HCD-AS zebrafish and HCD+LPS-AS zebrafish contained a large amount of lipids, while the lipid components of LPS-AS zebrafish plaques were few. Representative images were expressed, n=4.

Figure 4

Effects of atorvastatin, aspirin, and vitamin C on early symptoms of AS zebrafish. (a) Effects of atorvastatin, aspirin, and vitamin C on the cholesterol accumulation. (b) Effects of atorvastatin, aspirin, and vitamin C on the numbers of macrophages. Representative images were expressed, n=4.

Figure 5

Effects of atorvastatin, aspirin, and vitamin C on inflammation, lipid metabolism, and oxidative stress in early AS zebrafish. (a) Effects of atorvastatin, aspirin, and vitamin C on the numbers of neutrophils. (b) Effects of atorvastatin, aspirin, and vitamin C on the lipid level. (c) Effects of atorvastatin, aspirin, and vitamin C on the ROS activity. Representative images were expressed, n=4.

Figure 6

HE staining to detect the effects of atorvastatin, aspirin, and vitamin C on AS zebrafish plaque formation. Representative images were expressed, n=4.

Figure 7

EVG staining to detect the effects of atorvastatin, aspirin, and vitamin C on fibrous tissue in AS zebrafish plaques. Representative images were expressed, n=4.

Figure 8

Oil red O staining to detect the effects of atorvastatin, aspirin, and vitamin C on lipids in AS zebrafish plaques. Representative images were expressed, n=4.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oxid Med Cell Longev
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.