Xenotransplantation of leukemia cells into SCID zebrafish. (A) Microscopic observation of K562 cells at 5 dpf zebrafish xenograft. (B) The 5 dpf zebrafish xenograft embryos were dissociated and labeled leukemia cells were analyzed manually using a hemocytometer. Error bars were calculated based on biological replicates in the experiment. Quantitative values were means ± SEM from 3–4 independent groups of 10 individuals at 3 dpf and 5 dpf. Control at 2 dpf represented the initial transplanted cells into the zebrafish from two separate experiments and showed a consistent amount of transplanted cells. Significance p values were determined as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.

MOLM-13 cells in zebrafish xenografts and U0126 treatment. Proliferation of MOLM-13 cells in the zebrafish xenograft model with U0126 treatment started from 2 dpf. The zebrafish xenograft embryos were dissociated (control group at 2 dpf and 5 dpf and treatment group at 5 dpf), and labeled leukemia cells were analyzed by ImageXpress Pico. (A) Total MOLM-13 cells in zebrafish xenografts after U0126 treatment (0, 2.5, 5, and 10 µM). (B) Representative images of zebrafish xenografts at 5 dpf with U0126 treatments. Quantitative values were means ± SEM from 4 to 5 independent groups of 3 xenografts in each group. Significance p values were determined as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.

MV4-11 cells in zebrafish xenografts and drug treatment. Proliferation of MV4-11 cells in the zebrafish xenograft model with Ara-C and Aza treatments started from 2 dpf. The zebrafish xenograft embryos were dissociated (control group at 2 dpf and 5 dpf and treatment group at 5 dpf), and labeled leukemia cells were analyzed by ImageXpress Pico. (A) Total MV4-11 cells in zebrafish xenografts after Ara-C (500 µM), Aza (300 µM), and U0126 (10 µM) treatment. (B) Representative images of zebrafish xenografts with respective concentrations of drug treatment at 5 dpf. Quantitative values were means ± SEM from 5 to 8 independent groups of 3 xenografts in each group. Significance p values were determined as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.