Effects of ethanol exposure on zebrafish locomotor activity. After exposure to alcohol, we investigated the behavior of 120 hpf zebrafish larvae. (A) Total swimming distance in 10 min. (B) Swimming speed in 1 min. (C) Swimming time in 1 min. (D) Swimming distance in LD time intervals. (E) Total swimming distance in LD time intervals. Each group, n = 24. Student’s t-test was conducted. *p < 0.05; **p < 0.01; ***p < 0.001.

Effects of ethanol on HPA axis hormone levels in zebrafish larvae. Samples were taken at 3, 4, 5, and 6 dpf. (A) CRH levels from 3 to 6 dpf. (B) ACTH levels from 3 to 6 dpf. (C) Cortisol levels from 3 to 6 dpf. Three independent experiments were conducted. Student’s t-test was performed. *p < 0.05; **p < 0.01; ***p < 0.001.

HPA axis gene expression patterns in zebrafish larvae after ethanol exposure. Samples were taken at 3, 4, 5, and 6 dpf. The expression patterns of (A)crha, (B)crhb, (C)pomca, (D)pomcb, and (E)gr. Three independent experiments were conducted. Student’s t-test was performed. *p < 0.05; **p < 0.01; ***p < 0.001.

Level of neurotransmitters in zebrafish larvae after ethanol exposure. Samples were taken at 3, 4, 5, and 6 dpf. (A) Dopamine concentration from 3 to 6 dpf. (B) 5-HT concentration from 3 to 6 dpf. Three independent experiments were conducted. Student’s t-test was performed. *p < 0.05; **p < 0.01; ***p < 0.001.

Neurotransmitter metabolism gene expression patterns in zebrafish larvae after ethanol exposure. Samples were taken at 3, 4, 5, and 6 dpf. The expression patterns of (A)maoa, (B)th, and (C)dbh were observed. Three independent experiments were conducted. Student’s t-test was performed. *p < 0.05; **p < 0.01; ***p < 0.001.