- Title
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An Evolutionarily Conserved uORF Regulates PGC1α and Oxidative Metabolism in Mice, Flies, and Bluefin Tuna
- Authors
- Dumesic, P.A., Egan, D.F., Gut, P., Tran, M.T., Parisi, A., Chatterjee, N., Jedrychowski, M., Paschini, M., Kazak, L., Wilensky, S.E., Dou, F., Bogoslavski, D., Cartier, J.A., Perrimon, N., Kajimura, S., Parikh, S.M., Spiegelman, B.M.
- Source
- Full text @ Cell Metab.
Inhibitory PPARGC1A uORFs Are Functionally Conserved in Fly and Zebrafish but Not in Atlantic Bluefin Tuna (A) Top: schematic of GFP expression constructs that contain the mouse or fly PPARGC1A/spargel uORF. Bottom: constructs were transfected into Drosophila S2R+ cells, and GFP was monitored by microscopy. (B) Expression construct mRNA was injected into zebrafish zygotes and analyzed by fluorescence (images) or bioluminescence (graph). mCherry-Luc2 served as control for injection efficiency. N = 20; p < 0.01. (C) Alignment of conserved uORF upstream of fish ppargc1a orthologs. The uORF start codon is highlighted in red, whereas the PGC1α start codon is highlighted in green. (D) Expression construct mRNA was injected into zebrafish zygotes and analyzed by fluorescence (images) or bioluminescence (graph). mCherry-Luc2 served as control for injection efficiency. N = 20; p < 0.01. |
Reprinted from Cell Metabolism, 30, Dumesic, P.A., Egan, D.F., Gut, P., Tran, M.T., Parisi, A., Chatterjee, N., Jedrychowski, M., Paschini, M., Kazak, L., Wilensky, S.E., Dou, F., Bogoslavski, D., Cartier, J.A., Perrimon, N., Kajimura, S., Parikh, S.M., Spiegelman, B.M., An Evolutionarily Conserved uORF Regulates PGC1α and Oxidative Metabolism in Mice, Flies, and Bluefin Tuna, 190-200.e6, Copyright (2019) with permission from Elsevier. Full text @ Cell Metab.