CRISPR

CRISPR1-gpr182

ID

ZDB-CRISPR-210420-1

Name

CRISPR1-gpr182

Previous Names

None

Target

Sequence

5' - CTCCTGCAATACGGTTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns289	gpr182

Expression

Gene expression in Wild Types + CRISPR1-gpr182

No data available

Phenotype

Phenotype resulting from CRISPR1-gpr182

No data available

Phenotype of all Fish created by or utilizing CRISPR1-gpr182

Phenotype	Fish	Conditions	Figures
leukocyte increased amount, abnormal	gpr182^{bns289/bns289}	standard conditions	Fig. 5 from Kwon et al., 2020
spleen increased size, abnormal	gpr182^{bns289/bns289}	standard conditions	Fig. 5 from Kwon et al., 2020
lymphocyte decreased amount, abnormal	gpr182^{bns289/bns289}	standard conditions	Fig. 5 from Kwon et al., 2020
hematopoietic stem cell myb expression increased amount, abnormal	gpr182^{bns289/bns289}	standard conditions	Fig. 4 from Kwon et al., 2020
neutrophil increased amount, abnormal	gpr182^{bns289/bns289}	standard conditions	Fig. 5 from Kwon et al., 2020
negative regulation of hematopoietic stem cell differentiation decreased occurrence, abnormal	gpr182^{bns289/bns289}; la2Tg; s896Tg	standard conditions	Fig. 4 from Kwon et al., 2020
hematopoietic stem cell increased amount, abnormal	gpr182^{bns289/bns289}; la2Tg; s896Tg	standard conditions	Fig. 4 from Kwon et al., 2020
neutrophil increased amount, abnormal	gpr182^{bns289/bns289}; nz117Tg; s896Tg	standard conditions	Fig. 5 from Kwon et al., 2020
macrophage increased amount, abnormal	gpr182^{bns289/bns289}; ump2Tg	standard conditions	Fig. 5 from Kwon et al., 2020

Citations