Oxygen consumption rates (pmol min⁻¹ embryo⁻¹) in zebrafish embryos exposed to pyrovalerone for 24 hr. Each point represents a mean value ± SD of the mean (N = 5)

Locomotor analysis of distance moved over the 50 min during a visual motor response (VMR) test after 3‐hr treatment to pyrovalerone. Each graph (left and right) represents an independent experiment. Group mean of the distance‐moved (mm)‐per‐minute intervals for fish (right panel). Total distance moved in each interval of the light and dark. Data are presented as mean value ± SE. Sample sizes for each experiment are reported in the methods. Different letters denote significant differences among groups within an interval (p < .05)

Locomotor analysis of distance moved over the 50 min during a visual motor response (VMR) test after 24‐hr treatment to pyrovalerone. Group mean of the distance‐moved (mm)‐per‐minute intervals for fish (right panel). Total distance moved in each interval of the light and dark. Data are presented as mean value ± SE. Sample size is reported in the methods. Different letters denote significant differences among groups within an interval (p < .05)

The expression levels of dat1 mRNA. Data are presented as mean value ± standard error (N = 8–10). Different letters reflect a significant difference between the groups (p ≤ .05)