Effects of NAC on 6-OHDA-induced locomotor behavior deficits in zebrafish larvae.(A) Total distance travelled, (B) mean speed, (C) maximum acceleration, (D) absolute turn angle, (E) immobility time and (F) representative color heatmap of the behavior of one larvae from each treatment group during the trial 5 min duration. Data are expressed as mean ± standard error of mean (S.E.M.). n = 8–11. Two-way ANOVA followed by Bonferroni post hoc test. Study sites: CTRL, control; NAC, N-acetylcysteine; 6-OHDA, 6-hydroxydopamine. **p < 0.01, ***p < 0.001 vs. control group (6-OHDA-).

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Effects of NAC on 6-OHDA-induced morphological alterations in larvae zebrafish.(A) Total length, (B) head length, (C) forebrain width, (D) midbrain width, (E) eyes distance and (F) pictures of a larva from each treatment group. The arrow indicates a notable morphological alteration in the shape of the larvae. Data are expressed as mean ± standard error of mean (S.E.M.). n = 11–12. Two-way ANOVA followed by Bonferroni post hoc test. Study sites: CTRL, control; NAC, N-acetylcysteine, 1 mg/L; 6-OHDA, 6-hydroxydopamine, 250 μM. ***p < 0.001 vs. control group (6-OHDA-). Image credit/source: (F) Radharani Benvenutti.