FIGURE SUMMARY
Title

Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging

Authors
Kumar, M., Kishore, S., Nasenbeny, J., McLean, D.L., Kozorovitskiy, Y.
Source
Full text @ Opt. Express
Fig. 7

Imaging zebrafish larvae. (a) A high resolution confocal imaging of zebrafish cerebellum in nacre Tg(Olig2:GFP) fish acquired in 20 min. (b) The same cerebellar region imaged with 2P SOPi setup in 6 seconds. (c) The same cerebellar region imaged using 1P SOPi setup in 1 second. (d) Schematic diagram showing the arrangement for rapid volumetric GCaMP imaging of Tg(VGlut2a:Gal4;UAS:GCaMP6s) zebrafish hind-brain and spinal cord using a fast scanning 1P light-sheet in the SOPi setup. The volume scan consists of 10 segments, covered at rate of 100 fps leading to 10 VPS scan speed. (e) Left, GCaMP fluorescence in a subset of active cells during spontaneous activity, shown as standard deviation based intensity projections of the frames corresponding to slice position I and VIII in scanned volume (I-X). Right, GCaMP imaging traces corresponding to neurons 1-10 in optical sections I and VIII (30 sec).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Opt. Express
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