Determination of pharmacological injury doses—1 nL injection at 3 dpf.(A) Representative images showing pericardial edema (arrows) and remaining yolk reserves (*) presented in a portion of larvae administered adriamycin or puromycin. (B) Five milligrams per milliliter puromycin does not result in edema. Increasing the puromycin dose to 25 mg mL⁻¹ causes edema in >40% of fish from 24 hpi (mean ± SEM). (C) Four milligrams per milliliter adriamycin causes edema in >75% of larvae by 24 hpi (mean ± SEM). SEM, standard error of the mean; dpf, days postfertilization; hpi, hours postinjection.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Effect of intravenous puromycin injection at 3 dpf on GFB structure (n = 3–4).(A) Image analysis of GFB for determination of podocyte effacement. Black double line = total length of GBM. White lines = foot process width. (B, C) Representative transmission electron microscopy micrographs at 24 hpi showing normal GFB structure with primary (*) and secondary foot processes with slit diaphragms (arrows). Foot process morphology is not significantly different from that of sham fish at 8 hpi (data not shown). (D, E) 25 mg mL⁻¹ puromycin results in patchy podocyte effacement (white brackets). (F) Administration of 25 mg mL⁻¹ puromycin significantly decreases the mean (±SEM) number of foot processes per unit length of GBM (p = 0.005) and (G) mean (±SEM) foot process width (p = 0.04). Analysis by one-way ANOVA with post hoc Dunnet's test. Scale bars are all 0.5 μm. GFB, glomerular filtration barrier; GBM, glomerular basement membrane. p-value summary: * < 0.05; ** ≤ 0.002.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.