(A) Radial immuno assay. Serum from (i) uninfected control, (ii) control group-treated with jacalin alone, (iii) infected with A. hydrophila and treated with jacalin, (iv) infected with A. hydrophila alone, (v) infected with A. hydrophila and treated with JPtNPs. (B) Re-sensitization radial immuno assay. Serum collected from: (i) infected control, (ii) rescued zebrafish given second infection after three days, (iii) rescued zebrafish given third infection on day seven, (iv) third time infected and rescued fish after 21 days and (v) rescued fish from the third time infection was infected again for the fourth time on day 21. Representative result of assay repeated thrice with different samples. Rescued zebrafish refers to infected and JPtNPs treated animal and this is the first infection.

In vivo antibacterial activity. (A) infected group (B) infected fish treated after 3 h with 10 L of 0.05 mM JPtNPs

Acknowledgments
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