Effect of tanshinone IIA on lipid accumulation in zebrafish. Two day post fertilization (dpf) larvae were exposed in the absence (DMSO) or presence of tanshinone IIA at the indicated concentrations. After 24 h, three dpf larvae were stained with LipidGreen2 and visualized under bright field (A) and fluorescence field (B); (C,D) Size and intensity of yolk (white dash in B) were quantified using the ImageJ software, respectively. Graph bars indicate mean ± SEM (standard error of the mean). Unpaired t-test was used to analyze difference versus DMSO group, and statistical significance was set 0.05 and 0.01 (* p ≤ 0.05, ** p ≤ 0.01). n = 10.