FIGURE SUMMARY
Title

Dynamic expression analysis of armc10, the homologous gene of human GPRASP2, in zebrafish embryos

Authors
Liu, C., Lin, C., Yao, J., Wei, Q., Xing, G., Cao, X.
Source
Full text @ Mol. Med. Rep.
Fig. 1

Amino acid sequence alignment of armc10 from several species. Sequences were aligned using ClustalX2. The armadillo domains are indicated by underlining. Conserved residues are shown in black (100% conservation), dark grey (80% conservation) and light grey (60% conservation). An absence of shading denotes residues with 60% conservation. armc10, armadillo repeat containing 10.

Fig. 2

Analysis of conservative structure of protein functional domains. The ARM superfamily was present in the zebrafish Armc10 and Homo ARMCX3 sequences. Armc10, armadillo repeat-containing 10; ARMCX3, armadillo repeat-containing X-linked protein 3.

Fig. 3

Phylogenetic tree of the zebrafish ARMC10 amino acid sequence with other species. A phylogenetic tree was constructed using the neighbor-joining method with Mega 6.0. Species belonging to same class were labeled in the same color: Pink, mammalia; violet, actinopterygii; green, reptilian; blue, aves; brown, amphibia. The ARMC10 sequences were retrieved from the GenBank database. ARMC10, armadillo repeat containing 10.

Fig. 4

Amino acid sequence alignment of human GPRASP2 and other proteins. (A) Human GPRASP2 with human ARMCX3; (B) human ARMCX3 with zebrafish armc10; (C) human GPRASP2 with zebrafish Armc10. The same amino acids are marked in yellow. GPRASP2; G protein-coupled receptor-associated sorting protein 2; ARMCX3, armadillo repeat-containing X-linked protein 3; Armc10, armadillo repeat-containing 10.

Fig. 5

Temporal expression of zebrafish armc10. Temporal expression of zebrafish armc10 was analyzed using reverse transcription-polymerase chain reaction analysis. Zebrafish armc10 was first detected at the two-cell stage and persisted throughout development. β-actin was used as a control. armc10, armadillo repeat-containing 10.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 2-cell to Protruding-mouth
Fig. 6

Expression of armc10 during early embryonic development of zebrafish. Dorsal views at (A) cleavage stage (two-cell), (B) 75% epiboly, (C) 12 hpf, (D) 24 hpf, (E) 48 hpf. Views at (F) 72 hpf (left, lateral view; right, dorsal view) and (G) 96 hpf (left, lateral view; right, dorsal view). armc10, armadillo repeat-containing 10.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 2-cell to Day 4
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Med. Rep.
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