Sub-lethal effects after exposure to field water samples or to reconstituted metal mixtures. Length of the larvae (A) exposed to field water samples or (B) to reconstituted metal mixtures. Percentages of swim bladder inflation of all hatched larvae at 120 hpf (C) after exposure to field water samples, and (D) after exposure to reconstituted mixtures. Representative photograph: the upper larva has an inflated swim bladder and the bottom one has a non-inflated swim bladder. Average swimming distance of (E) larvae exposed to field water samples, and (F) to reconstituted metal mixtures. For an explanation of the controls, see Figure 2. Different small letters indicate significant differences (p < 0.05) (n is given in parentheses).