- Title
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Excitation-contraction coupling in zebrafish ventricular myocardium is regulated by trans-sarcolemmal Ca2+ influx and sarcoplasmic reticulum Ca2+ release
- Authors
- Haustein, M., Hannes, T., Trieschmann, J., Verhaegh, R., Köster, A., Hescheler, J., Brockmeier, K., Adelmann, R., Khalil, M.
- Source
- Full text @ PLoS One
Preparation and histology of zebrafish ventricular myocardial tissue slices. (A) Excised zebrafish heart. Slices were sectioned along the short axis as displayed by the sectional plane. (B) Picture of a myocardial tissue slice. Dashed circle encloses the ventricular cavity used for mounting on the force setup. (C) Technique used for mounting of tissue slices. The slice is impaled on a glass capillary with a conic tip. The lumen of the capillary is placed on the tips of the J-shaped hooks and the tissue slice is pushed down. Afterwards the hooks with the slice are lowered into the medium-containing measuring chamber. (D-M) Histological and immunohistochemical stainings of myocardial tissue slices. HE staining of cryosections (8 µm) obtained from a myocardial tissue slice at day 0 (D), day 1 (G) and day 2 (K). Cyrosections stained against sarcomeric-α-actinin (green) at day 0 (E-F), day 1 (H-I) and day 2 (L-M). Nuclei are counterstained with Hoechst (blue). |
Characterisation of zebrafish ventricular myocardial slices after prolonged culture and cultured under hypoxic conditions. HE staining of cryosections (8 µm) obtained from a myocardial tissue slice at day 4 (A) and cultured under hypoxic conditions (1% O2 for 2 days) (D). Cyrosections stained against sarcomeric-α-actinin (green) at day 4 (B-C) or cultured under hypoxic conditions (E-F). Nuclei are counterstained with Hoechst (blue). |