Fig. 1

Nherf1 and Planar Cell Polarity. (A and B) Live imaging of zebrafish ionocytes using ConA-FITC and MitoTracker red labeling in wildtype and knypek (kny-/-) mutants. Top panels show a view of a single cell on the surface of the yolk sac, bottom panels show a side view of the z-stack depicting apical trafficking. (C and D) Phalloidin-Cy3 staining of zebrafish epidermal cells (keratinocytes) in fixed embryos from uninjected wildtype and nherf1 MO injected animals. (E and F) Staining in kny-/- animals, either uninjected (E) or injected with the nherf1 MO (F).

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