ZFIN ID: ZDB-LAB-070718-1
National BioResource Project, Zebrafish, NBRP/Brain Science Institute, RIKEN
PI/Director: Okamoto, Hitoshi
Contact Person: Ishioka, Akiko
Email: hitoshi@brain.riken.go.jp
URL: http://www.shigen.nig.ac.jp/zebra/index_en.html
Address: Brain Science Institute, RIKEN 2-1, Hirosawa Wakou-shi Saitama, 351-0198
Phone: 048-467-9712
Fax: 048-467-9714
Line Designation: None assigned


GENOMIC FEATURES ORIGINATING FROM THIS LAB

STATEMENT OF RESEARCH INTERESTS


LAB MEMBERS


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Chou, M.Y., Amo, R., Kinoshita, M., Cherng, B.W., Shimazaki, H., Agetsuma, M., Shiraki, T., Aoki, T., Takahoko, M., Yamazaki, M., Higashijima, S., Okamoto, H. (2016) Social conflict resolution regulated by two dorsal habenular subregions in zebrafish. Science (New York, N.Y.). 352:87-90
Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A. (2015) Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Scientific Reports. 5:8841
Amo, R., Fredes, F., Kinoshita, M., Aoki, R., Aizawa, H., Agetsuma, M., Aoki, T., Shiraki, T., Kakinuma, H., Matsuda, M., Yamazaki, M., Takahoko, M., Tsuboi, T., Higashijima, S.I., Miyasaka, N., Koide, T., Yabuki, Y., Yoshihara, Y., Fukai, T., Okamoto, H. (2014) The Habenulo-Raphe Serotonergic Circuit Encodes an Aversive Expectation Value Essential for Adaptive Active Avoidance of Danger. Neuron. 84:1034-1048
Aoki, R., Tsuboi, T., Okamoto, H. (2014) Y-maze avoidance: An automated and rapid associative learning paradigm in zebrafish. Neurosciences research. 91:69-72
Okamoto, H. (2014) Neurobiology: sensory lateralization in the fish brain. Current biology : CB. 24:R285-7
Nishiya, N., Oku, Y., Kumagai, Y., Sato, Y., Yamaguchi, E., Sasaki, A., Shoji, M., Ohnishi, Y., Okamoto, H., Uehara, Y. (2014) A Zebrafish Chemical Suppressor Screening Identifies Small Molecule Inhibitors of the Wnt/beta-catenin Pathway. Chemistry & Biology. 21:530-40
Aoki, M., Segawa, H., Naito, M., and Okamoto, H. (2014) Identification of possible downstream genes required for the extension of peripheral axons in primary sensory neurons. Biochemical and Biophysical Research Communications. 445(2):357-362
Campbell, D.S., and Okamoto, H. (2013) Local caspase activation interacts with Slit-Robo signaling to restrict axonal arborization. The Journal of cell biology. 203(4):657-672
Mizuno, H., Sassa, T., Higashijima, S.I., Okamoto, H., and Miyawaki, A. (2013) Transgenic zebrafish for ratiometric imaging of cytosolic and mitochondrial Ca2+ response in teleost embryo. Cell Calcium. 54(3):236-45
Aoki, T., Kinoshita, M., Aoki, R., Agetsuma, M., Aizawa, H., Yamazaki, M., Takahoko, M., Amo, R., Arata, A., Higashijima, S.I., Tsuboi, T., and Okamoto, H. (2013) Imaging of Neural Ensemble for the Retrieval of a Learned Behavioral Program. Neuron. 78(5):881-894
Matsui, T., Thitamadee, S., Murata, T., Kakinuma, H., Nabetani, T., Hirabayashi, Y., Hirate, Y., Okamoto, H., and Bessho, Y. (2011) Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis. Proc. Natl. Acad. Sci. USA. 108(24):9881-6
Okamoto, H., Agetsuma, M., and Aizawa, H. (2011) Genetic dissection of the zebrafish habenula, a possible switching board for selection of behavioral strategy to cope with fear and anxiety. Developmental Neurobiology. 72(3):386-394
Ohata, S., Aoki, R., Kinoshita, S., Yamaguchi, M., Tsuruoka-Kinoshita, S., Tanaka, H., Wada, H., Watabe, S., Tsuboi, T., Masai, I., and Okamoto, H. (2011) Dual Roles of Notch in Regulation of Apically Restricted Mitosis and Apicobasal Polarity of Neuroepithelial Cells. Neuron. 69(2):215-230
Tanaka, H., Nojima, Y., Shoji, W., Sato, M., Nakayama, R., Ohshima, T., and Okamoto, H. (2011) Islet1 selectively promotes peripheral axon outgrowth in Rohon-Beard primary sensory neurons. Developmental dynamics : an official publication of the American Association of Anatomists. 240(1):9-22
Agetsuma, M., Aizawa, H., Aoki, T., Nakayama, R., Takahoko, M., Goto, M., Sassa, T., Amo, R., Shiraki, T., Kawakami, K., Hosoya, T., Higashijima, S.I., and Okamoto, H. (2010) The habenula is crucial for experience-dependent modification of fear responses in zebrafish. Nature Neuroscience. 13(11):1354-1356
Ito, Y., Tanaka, H., Okamoto, H., and Ohshima, T. (2010) Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum. Developmental Biology. 342(1):26-38
Amo, R., Aizawa, H., Takahoko, M., Kobayashi, M., Takahashi, R., Aoki, T., and Okamoto, H. (2010) Identification of the zebrafish ventral habenula as a homolog of the mammalian lateral habenula. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(4):1566-1574
Okamoto, H., and Ishioka, A. (2010) Zebrafish Research in Japan and the National BioResource Project. Experimental animals. 59(1):9-12
Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S.I., and Miyawaki, A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA. 106(49):20812-20817
Ohata, S., Kinoshita, S., Aoki, R., Tanaka, H., Wada, H., Tsuruoka-Kinoshita, S., Tsuboi, T., Watabe, S., and Okamoto, H. (2009) Neuroepithelial cells require fucosylated glycans to guide the migration of vagus motor neuron progenitors in the developing zebrafish hindbrain. Development (Cambridge, England). 136(10):1653-1663
Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., and Yoshihara, Y. (2009) From the olfactory bulb to higher brain centers: genetic visualization of secondary olfactory pathways in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(15):4756-4767
Wada, H., and Okamoto, H. (2009) Roles of planar cell polarity pathway genes for neural migration and differentiation. Development, Growth & Differentiation. 51(3):233-240
Wada, H., and Okamoto, H. (2009) Roles of Noncanonical Wnt/PCP Pathway Genes in Neuronal Migration and Neurulation in Zebrafish. Zebrafish. 6(1):3-8
Carreira-Barbosa, F., Kajita, M., Morel, V., Wada, H., Okamoto, H., Martinez Arias, A., Fujita, Y., Wilson, S.W., and Tada, M. (2009) Flamingo regulates epiboly and convergence/extension movements through cell cohesive and signalling functions during zebrafish gastrulation. Development (Cambridge, England). 136(3):383-392
Nishiwaki, Y., Komori, A., Sagara, H., Suzuki, E., Manabe, T., Hosoya, T., Nojima, Y., Wada, H., Tanaka, H., Okamoto, H., and Masai, I. (2008) Mutation of cGMP phosphodiesterase 6alpha'-subunit gene causes progressive degeneration of cone photoreceptors in zebrafish. Mechanisms of Development. 125(11-12):932-946
Okamoto, H., Sato, T., and Aizawa, H. (2008) Transgenic technology for visualization and manipulation of the neural circuits controlling behavior in zebrafish. Development, Growth & Differentiation. 50(s1):S167-S175
Yamaguchi, M., Fujimori-Tonou, N., Yoshimura, Y., Kishi, T., Okamoto, H., and Masai, I. (2008) Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53. Development (Cambridge, England). 135(7):1247-1257
Omata, Y., Nojima, Y., Nakayama, S., Okamoto, H., Nakamura, H., and Funahashi, J.I. (2007) Role of Bone morphogenetic protein 4 in zebrafish semicircular canal development. Development, Growth & Differentiation. 49(9):711-719
Okamoto, H. (2007) Yin-yang ways of controlling gene expression are now in our hands. ACS Chemical Biology. 2(10):646-648
Tanaka, H., Maeda, R., Shoji, W., Wada, H., Masai, I., Shiraki, T., Kobayashi, M., Nakayama, R., and Okamoto, H. (2007) Novel mutations affecting axon guidance in zebrafish and a role for plexin signalling in the guidance of trigeminal and facial nerve axons. Development (Cambridge, England). 134(18):3259-3269
Sato, T., Hamaoka, T., Aizawa, H., Hosoya, T., and Okamoto, H. (2007) Genetic single-cell mosaic analysis implicates ephrinB2 reverse signaling in projections from the posterior tectum to the hindbrain in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(20):5271-5279
Sassa, T., Aizawa, H., and Okamoto, H. (2007) Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems. Developmental dynamics : an official publication of the American Association of Anatomists. 236(3):706-718
Aizawa, H., Goto, M., Sato, T., and Okamoto, H. (2007) Temporally regulated asymmetric neurogenesis causes left-right difference in the zebrafish habenular structures. Developmental Cell. 12(1):87-98
Wada, H., Tanaka, H., Nakayama, S., Iwasaki, M., and Okamoto, H. (2006) Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain. Development (Cambridge, England). 133(23):4749-4759
Sato, T., Takahoko, M., and Okamoto, H. (2006) HuC:Kaede, a useful tool to label neural morphologies in networks in vivo. Genesis (New York, N.Y. : 2000). 44(3):136-142
Hirate, Y., and Okamoto, H. (2006) Canopy1, a Novel Regulator of FGF Signaling around the Midbrain-Hindbrain Boundary in Zebrafish. Current biology : CB. 16(4):421-427
Ando, H., Kobayashi, M., Tsubokawa, T., Uyemura, K., Furuta, T., and Okamoto, H. (2005) Lhx2 mediates the activity of Six3 in zebrafish forebrain growth. Developmental Biology. 287(2):456-468
Yamaguchi, M., Tonou-Fujimori, N., Komori, A., Maeda, R., Nojima, Y., Li, H., Okamoto, H., and Masai, I. (2005) Histone deacetylase 1 regulates retinal neurogenesis in zebrafish by suppressing Wnt and Notch signaling pathways. Development (Cambridge, England). 32(13):3027-3043
Wada, H., Iwasaki, M., Sato, T., Masai, I., Nishiwaki, Y., Tanaka, H., Sato, A., Nojima, Y., and Okamoto, H. (2005) Dual roles of zygotic and maternal Scribble1 in neural migration and convergent extension movements in zebrafish embryos. Development (Cambridge, England). 132(10):2273-2285
Masai, I., Yamaguchi, M., Tonou-Fujimori, N., Komori, A., and Okamoto, H. (2005) The hedgehog-PKA pathway regulates two distinct steps of the differentiation of retinal ganglion cells: the cell-cycle exit of retinoblasts and their neuronal maturation. Development (Cambridge, England). 132(7):1539-1553
Kawakami, A., Nojima, Y., Toyoda, A., Takahoko, M., Satoh, M., Tanaka, H., Wada, H., Masai, I., Terasaki, H., Sakaki, Y., Takeda, H., and Okamoto, H. (2005) The zebrafish-secreted matrix protein you/scube2 is implicated in long-range regulation of hedgehog signaling. Current biology : CB. 15(5):480-488
Miyasaka, N., Sato, Y., Yeo, S.Y., Hutson, L.D., Chien, C.B., Okamoto, H., and Yoshihara, Y. (2005) Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system. Development (Cambridge, England). 132(6):1283-1293
Li, Q., Shirabe, K., Thisse, C., Thisse, B., Okamoto, H., Masai, I., and Kuwada, J.Y. (2005) Chemokine signaling guides axons within the retina in zebrafish. The Journal of neuroscience : the official journal of the Society for Neuroscience. 25(7):1711-1717
Emoto, Y., Wada, H., Okamoto, H., Kudo, A., and Imai, Y. (2005) Retinoic acid-metabolizing enzyme Cyp26a1 is essential for determining territories of hindbrain and spinal cord in zebrafish. Developmental Biology. 278(2):415-427
Uemura, O., Okada, Y., Ando, H., Guedj, M., Higashijima, S., Shimazaki, T., Chino, N., Okano, H., and Okamoto, H. (2005) Comparative functional genomics revealed conservation and diversification of three enhancers of the isl1 gene for motor and sensory neuron-specific expression. Developmental Biology. 278(2):587-606
Aizawa, H., Bianco, I.H., Hamaoka, T., Miyashita, T., Uemura, O., Concha, M.L., Russell, C., Wilson, S.W., and Okamoto, H. (2005) Laterotopic Representation of Left-Right Information onto the Dorso-Ventral Axis of a Zebrafish Midbrain Target Nucleus. Current biology : CB. 15(3):238-243
Ando, H., Furuta, T., and Okamoto, H. (2004) Photo-mediated gene activation by using caged mRNA in zebrafish embryos. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:159-171
Hanaoka, R., Ohmori, Y., Uyemura, K., Hosoya, T., Hotta, Y., Shirao, T., and Okamoto, H. (2004) Zebrafish gcmb is required for pharyngeal cartilage formation. Mechanisms of Development. 121(10):1235-1247
Miyashita, T., Yeo, S.Y., Hirate, Y., Segawa, H., Wada, H., Little, M.H., Yamada, T., Takahashi, N., and Okamoto, H. (2004) PlexinA4 is necessary as a downstream target of Islet2 to mediate Slit signaling for promotion of sensory axon branching. Development (Cambridge, England). 131(15):3705-3715
Yeo, S.Y., Miyashita, T., Fricke, C., Little, M.H., Yamada, T., Kuwada, J.Y., Huh, T.L., Chien, C.B., and Okamoto, H. (2004) Involvement of Islet-2 in the Slit signaling for axonal branching and defasciculation of the sensory neurons in embryonic zebrafish. Mechanisms of Development. 121(4):315-324
Okamoto, H., Hirate, Y., and Ando, H. (2004) Systematic identification of factors in zebrafish regulating the early midbrain and cerebellar development by odered differential display and caged mRNA technology. Frontiers in bioscience : a journal and virtual library. 9:93-99
Ando, H. and Okamoto, H. (2003) Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA. Methods in cell science : an official journal of the Society for In Vitro Biology. 25(1-2):25-31
Hutson, L.D., Jurynec, M.J., Yeo, S.Y., Okamoto, H., and Chien, C.B. (2003) Two divergent slit1 genes in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 228(3):358-369
Hirate, Y., Okamoto, H. and Yamasu, K. (2003) Structure of the zebrafish fasciclin I-related extracellular matrix protein (betaig-h3) and its characteristic expression during embryogenesis. Gene Expression Patterns. 3(3):331-336
Masai, I., Lele, Z., Yamaguchi, M., Komori, A., Nakata, A., Nishiwaki, Y., Wada, H., Tanaka, H., Nojima, Y., Hammerschmidt, M., Wilson, S.W., and Okamoto, H. (2003) N-cadherin mediates retinal lamination, maintenance of forebrain compartments and patterning of retinal neurites. Development (Cambridge, England). 130(11):2479-2494
Zeller, J., Schneider, V., Malayaman, S., Higashijima, S., Okamoto, H., Gui, J., Lin, S., and Granato, M. (2002) Migration of zebrafish spinal motor nerves into the periphery requires multiple myotome-derived cues. Developmental Biology. 252(2):241-256
Bingham, S., Higashijima, S.-I., Okamoto, H., and Chandrasekhar, A. (2002) The zebrafish trilobite gene is essential for tangential migration of branchiomotor neurons. Developmental Biology. 242(2):149-160
Ando, H., Okamoto, H., and Furuta, T. (2002) Development of a new technology to manipulate gene activity by photo-illumination using a new DNA/RNA caging compound, Bhc-diazo. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 47(2):125-132
Hirate, Y., Mieda, M., Harada, T., Yamasu, K., and Okamoto, H. (2001) Identification of ephrin-A3 and novel genes specific to the midbrain-MHB in embryonic zebrafish by ordered differential display. Mechanisms of Development. 107(1-2):83-96
Okamoto, H.M., Nakayama, I., Nagoya, H., and Araki, K. (2001) Predicted protein structure of medaka FoxA3 and its expression in polster. Zool. Sci.. 18(6):823-832
Mizuno, T., Kawasaki, M., Nakahira, M., Kagamiyama, H., Kikuchi, Y., Okamoto, H., Mori, K., and Yoshihara, Y. (2001) Molecular diversity in zebrafish NCAM family: three members with different VASE usage and distinct localization. Molecular and Cellular Neuroscience. 18(1):119-130
Segawa, H., Miyashita, T., Hirate, Y., Higashijima, S., Chino, N., Uyemura, K., Kikuchi, Y., and Okamoto, H. (2001) Functional repression of islet-2 by disruption of complex with Ldb impairs peripheral axonal outgrowth in embryonic zebrafish. Neuron. 30(2):423-436
Yeo, S.Y., Little, M.H., Yamada, T., Miyashita, T., Halloran, M.C., Kuwada, J.Y., Huh, T.L., and Okamoto, H. (2001) Overexpression of a slit homologue impairs convergent extension of the mesoderm and causes cyclopia in embryonic zebrafish. Developmental Biology. 230(1):1-17
Wada, H., Okamoto, H., and Higashijima, S. (2000) [Study of mechanisms for the axonal pathfinding of the trigeminal motor neurons by using transgenic zebrafish].. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 45(Suppl. 17):2803-2809
Masai, I., Stemple, D.L., Okamoto, H., and Wilson, S.W. (2000) Midline signals regulate retinal neurogenesis in zebrafish. Neuron. 27(2):251-263
Okamoto, H., Kikuchi, H., and Segawa, H. (2000) Islet-1 family and neural differentiation: study using zebrafish embryos. (in Japanese). Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. (Suppl. 3) 45:233-240
Higashijima, S., Hotta, Y., and Okamoto, H. (2000) Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer. The Journal of neuroscience : the official journal of the Society for Neuroscience. 20(1):206-218
Saito, T., Hascilowicz, T., Ohkido, I., Kikuchi, Y., Okamoto, H., Hayashi, S., Murakami, Y., and Matsufuji, S. (2000) Two zebrafish (Danio rerio) antizymes with different expression and activities. The Biochemical journal. 345(1):99-106
Mieda, M., Kikuchi, Y., Hirate, Y., Aoki, M., and Okamoto, H. (1999) Compartmentalized expression of zebrafish ten-m3 and ten-m4, homologues of the Drosophila ten(m)/odd Oz gene, in the central nervous system. Mechanisms of Development. 87(1-2):223-227
Izsvák, Z., Ivics, Z., Shimoda, N., Mohn, D., Okamoto, H., and Hackett, P.B. (1999) Short inverted-repeat transposable elements in teleost fish and implications for a mechanism of their amplification. Journal of molecular evolution. 48:13-21
Higashijima, S., Nose, A., Eguchi, G., Hotta, Y., and Okamoto, H. (1997) Mindin/F-spondin family: novel ECM proteins expressed in the zebrafish embryonic axis. Developmental Biology. 192:211-227
Higashijima, S., Okamoto, H., Ueno, N., Hotta, Y., and Eguchi, G. (1997) High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin. Developmental Biology. 192:289-299
Kikuchi, Y., Segawa, H., Tokumoto, M., Tsubokawa, T., Hotta, Y., Uyemura, K., and Okamoto, H. (1997) Ocular and cerebellar defects in zebrafish induced by overexpression of the LIM domains of the islet-3 LIM/homeodomain protein. Neuron. 18(3):369-382
Kikuchi, Y., Segawa, H., Tokumoto, M., Hotta, Y., Uyemura, K., and Okamoto, H. (1997) Functional analysis of zebrafish Islet-3. Neurosciences research.
Segawa, H., Kikuchi, Y., Chino, N., Uyemura, K., and Okamoto, H. (1997) Peripheral-branch specific control of axonogenesis in zebrafish sensory neurons by LIM/homeodomain proteins, Islet-2 and Islet-3. Neurosciences research. :7-29
Kikuchi, Y., Tokumoto, M., Hotta, Y., Uyemura, K., and Okamoto, H. (1996) Functional analysis of zebrafish Islet-1 protein family. Neurosciences research. 25 sup. 2:116
Kim, C.H., Taira, E., Kuo, C.H., Li, B.S., Okamoto, H., Nakahira, K., Ikenaka, K., Higuchi, H., and Miki, N. (1996) Neuron-specific expression of a chicken gicerin cDNA transient transgenic zebrafish. Neurochemical research. 21:231-237
Shimoda, N., Chevrette, M., Ekker, M., Kikuchi, Y., Hotta, Y., and Okamoto, H. (1996) Mermaid, a family of short interspersed repetitive elements, is useful for zebrafish genome mapping. Biochemical and Biophysical Research Communications. 220:233-237
Kuo, C.H., Uetsuki, T., Kim, C.H., Tanaka, H., Li, B.S., Taira, E., Higuchi, H., Okamoto, H., Yoshikawa, K., and Miki, N. (1995) Determination of a necdin cis-acting element required for neuron specific expression by using zebra fish. Biochemical and Biophysical Research Communications. 211(2):438-446
Tokumoto, M., Gong, Z.Y., Tsubokawa, T., Hew, C.L., Uyemura, K., Hotta, Y., and Okamoto, H. (1995) Molecular heterogeneity among primary motoneurons and within myotomes revealed by the differential mRNA expression of novel islet-1 homologs in embryonic zebrafish. Developmental Biology. 171:578-589
Inoue, A., Takahashi, M., Hatta, K., Hotta, Y., and Okamoto, H. (1994) Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 199:1-11