ZFIN ID: ZDB-LAB-001207-1
Martial Lab
PI/Director: Martial, Joseph A.
Co-PI / Senior
Researcher:
Muller, Marc
Peers, Bernard
Contact Person: Muller, Marc
Email: M.Muller@ulg.ac.be
URL: http://www.lbmgg.ulg.ac.be/index.php?option=com_content&view=frontpage&Itemid=1
Address: Laboratory of Molecular Biology and Genetic Engineering Institute of Chemistry University of Liège Allée du 6 Août Bâtiment B6 B-4000 Liège (Sart Tilman) BELGIUM
Country: Belgium
Phone: 32-4-366-4437
Fax: 32-4-366-2968
Line Designation: ulg


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 3 genomic features


STATEMENT OF RESEARCH INTERESTS
(1) Molecular mechanisms controlling pancreas organogenesis and differentiation of pancreatic cells. Project leader: Bernard Peers

The goal of this project is to identify and study regulatory genes involved in the differentiation of pancreatic cells. Using zebrafish as animal model, we are studying the role of several transcription factors (i.e. Meis, Pbx, SOX4 factors) in pancreas development. The expression of these factors has been analysed during zebrafish embryogenesis by in situ hybridization. The role of these factors is studied by loss and gain of function strategies (injection of mRNA and antisens morphonino into zebrafish fertilized eggs). We have also generated zebrafish transgenic lines expressing GFP under the control of the HB9 and Pax6 promoters which are active in endocrine pancreatic cells. Observation of living transgenic embryos under fluorescent microscope allow to follow the formation of the endocrine islet. We are using these transgenic lines to perform a genetic screenning for zebrafish mutants having endocrine pancreatic defects.


(2) Molecular dissection of the role of the PLAG family members in oncogenesis and embryogenesis using the zebrafish as animal model. Project leader: Marianne Voz

PLAG gene family comprise three members, PLAG1, PLAGL1 and PLAGL2 which all seem to be involved in tumor formation. Moreover, PLAG1 and PLAGL2 are mainly expressed during fetal life suggesting important functions during development. To determine their physiological role, we analyse the expression pattern of the PLAG factors during zebrafish development by whole mount in situ and test their role by gain or loss function strategies(injection of mRNAs or of morpholinos/siRNAs). Transgenic approach to ectopically overexpress these factors will evaluate their oncogenic
potential in zebrafish.


(3) Study of the antiangiogenic factor 16K hPRL: understanding of its mechanism of action and evaluation of its therapeutic potential. Project leader: Ingrid Struman.

Our group is studying the 16 kDa N-terminal fragment of human prolactin, also named 16K hPRL that has been shown to be antiangiogenic in vitro and in vivo. Research conducted in our lab attempts to:
* Understand the molecular mechanisms by which 16K hPRL induces apoptosis and inhibits proliferation of endothelial cells.
* Identify the receptor involved in the antiangiogenic action of 16K hPRL.
* Search for minimal fragments of 16K hPRL that retain antiangiogenic activity.
* Determine the effect of 16K hPRL in early blood vessel development in zebrafish.
* Evaluate the potential use of 16K hPRL in an antiangiogenic therapy for cancer and other angiogenesis-related diseases.


(4) The organogenesis of the pituitary gland is characterized in the zebrafish and the regulatory factors governing this process in early embryonic development are investigated.


LAB MEMBERS
Manfroid, Isabelle Post-Doc Pendeville-Samain, Hélène Post-Doc Winandy, Marie Post-Doc
Binot, Anne-Catherine Graduate Student Delporte, Francois Graduate Student Lopez, Mauricio Graduate Student
Verbruggen, Vincianne Graduate Student Planchon, Liliane Fish Facility Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS x
Ledoux, A., St-Gelais, A., Cieckiewicz, E., Jansen, O., Bordignon, A., Illien, B., Di Giovanni, N., Marvilliers, A., Hoareau, F., Pendeville, H., Quetin-Leclercq, J., Frédérich, M. (2017) Antimalarial Activities of Alkyl Cyclohexenone Derivatives Isolated from the Leaves of Poupartia borbonica. J. Nat. Prod.. 80(6):1750-1757
Otten, A.B., Stassen, A.P., Adriaens, M., Gerards, M., Dohmen, R.G., Timmer, A.J., Vanherle, S.J., Kamps, R., Boesten, I.B., Vanoevelen, J.M., Muller, M., Smeets, B. (2016) Replication Errors Made During Oogenesis Lead to Detectable De Novo mtDNA Mutations in Zebrafish Oocytes with a Low mtDNA Copy Number. Genetics. 204(4):1423-1431
Moortgat, S., Désir, J., Benoit, V., Boulanger, S., Pendeville, H., Nassogne, M.C., Lederer, D., Maystadt, I. (2016) Two novel EIF2S3 mutations associated with syndromic intellectual disability with severe microcephaly, growth retardation, and epilepsy. American journal of medical genetics. Part A. 170(11):2927-2933
Verstraelen, S., Peers, B., Maho, W., Hollanders, K., Remy, S., Berckmans, P., Covaci, A., Witters, H. (2016) Phenotypic and biomarker evaluation of zebrafish larvae as an alternative model to predict mammalian hepatotoxicity. Journal of applied toxicology : JAT. 36(9):1194-206
Otten, A.B., Theunissen, T.E., Derhaag, J.G., Lambrichs, E.H., Boesten, I.B., Winandy, M., van Montfoort, A.P., Tarbashevich, K., Raz, E., Gerards, M., Vanoevelen, J.M., van den Bosch, B.J., Muller, M., Smeets, H.J. (2016) Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells. Cell Reports. 16(3):622-30
Halluin, C., Madelaine, R., Naye, F., Peers, B., Roussigné, M., Blader, P. (2016) Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade. PLoS One. 11:e0158210
Babayigit, A., Ethirajan, A., Muller, M., Conings, B. (2016) Toxicity of organometal halide perovskite solar cells. Nature Materials. 15:247-51
Babayigit, A., Duy Thanh, D., Ethirajan, A., Manca, J., Muller, M., Boyen, H.G., Conings, B. (2016) Assessing the toxicity of Pb- and Sn-based perovskite solar cells in model organism Danio rerio. Scientific Reports. 6:18721
Janssen, L., Dupont, L., Bekhouche, M., Noel, A., Leduc, C., Voz, M., Peers, B., Cataldo, D., Apte, S.S., Dubail, J., Colige, A. (2016) ADAMTS3 activity is mandatory for embryonic lymphangiogenesis and regulates placental angiogenesis. Angiogenesis. 19(1):53-65
Ishibashi, M., Manning, E., Shoubridge, C., Krecsmarik, M., Hawkins, T.A., Giacomotto, J., Zhao, T., Mueller, T., Bader, P.I., Cheung, S.W., Stankiewicz, P., Bain, N.L., Hackett, A., Reddy, C.C., Mechaly, A.S., Peers, B., Wilson, S.W., Lenhard, B., Bally-Cuif, L., Gecz, J., Becker, T.S., Rinkwitz, S. (2015) Copy number variants in patients with intellectual disability affect the regulation of ARX transcription factor gene. Human genetics. 134(11-12):1163-82
Ghaye, A.P., Bergemann, D., Tarifeño-Saldivia, E., Flasse, L.C., Von Berg, V., Peers, B., Voz, M.L., Manfroid, I. (2015) Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration. BMC Biology. 13:70
Aceto, J., Nourizadeh-Lillabadi, R., Marée, R., Dardenne, N., Jeanray, N., Wehenkel, L., Aleström, P., van Loon, J.J., Muller, M. (2015) Zebrafish Bone and General Physiology Are Differently Affected by Hormones or Changes in Gravity. PLoS One. 10:e0126928
Windhausen, T., Squifflet, S., Renn, J., Muller, M. (2015) BMP Signaling Regulates Bone Morphogenesis in Zebrafish through Promoting Osteoblast Function as Assessed by Their Nitric Oxide Production. Molecules. 20:7586-7601
Jeanray, N., Marée, R., Pruvot, B., Stern, O., Geurts, P., Wehenkel, L., Muller, M. (2015) Phenotype classification of zebrafish embryos by supervised learning. PLoS One. 10:e0116989
Renn, J., Pruvot, B., and Muller, M. (2014) Detection of nitric oxide by diaminofluorescein visualizes the skeleton in living zebrafish. Journal of Applied Ichthyology. 30(4):701-706
Pruvot, B., Curé, Y., Djiotsa, J., Voncken, A., and Muller, M. (2014) Developmental defects in zebrafish for classification of EGF pathway inhibitors. Toxicol. Appl. Pharmacol.. 274(2):339-349
Exertier, P., Javerzat, S., Wang, B., Franco, M., Herbert, J., Platonova, N., Winandy, M., Pujol, N., Nivelles, O., Ormenese, S., Godard, V., Becker, J., Bicknell, R., Pineau, R., Wilting, J., Bikfalvi, A., and Hagedorn, M. (2013) Impaired angiogenesis and tumor development by inhibition of the mitotic kinesin Eg5. Oncotarget. 4(12):2302-2316
Larbuisson, A., Dalcq, J., Martial, J.A., and Muller, M. (2013) Fgf receptors Fgfr1a and Fgfr2 control the function of pharyngeal endoderm in late cranial cartilage development. Differentiation; research in biological diversity. 86(4-5):192-206
Flasse, L.C., Pirson, J.L., Stern, D.G., Von Berg, V., Manfroid, I., Peers, B., and Voz, M.L. (2013) Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology. 11(1):78
Flasse, L., Stern, D.G., Pirson, J.L., Manfroid, I., Peers, B., and Voz, M.L. (2013) The bHLH transcription factor Ascl1a is essential for the specification of the intestinal secretory cells and mediates Notch signaling in the zebrafish intestine. Developmental Biology. 376(2):187-197
Djiotsa, J., Verbruggen, V., Giacomotto, J., Ishibashi, M., Manning, E., Rinkwitz, S., Manfroid, I., Lvoz, M., and Peers, B. (2012) Pax4 is not essential for beta-cell differentiation in zebrafish embryos but modulates alpha-cell generation by repressing arx gene expression. BMC Developmental Biology. 12(1):37
Pruvot, B., Quiroz, Y., Voncken, A., Jeanray, N., Piot, A., Martial, J.A., and Muller, M. (2012) A panel of biological tests reveals developmental effects of pharmaceutical pollutants on late stage zebrafish embryos. Reproductive toxicology (Elmsford, N.Y.). 34(4):568-583
Manfroid, I., Ghaye, A., Naye, F., Detry, N., Palm, S., Pan, L., Ma, T.P., Huang, W., Rovira, M., Martial, J.A., Parsons, M.J., Moens, C.B., Voz, M.L., and Peers, B. (2012) Zebrafish sox9b is crucial for hepatopancreatic duct development and pancreatic endocrine cell regeneration. Developmental Biology. 366(2):268-278
Quiroz, Y., Lopez, M., Mavropoulos, A., Motte, P., Martial, J.A., Hammerschmidt, M., and Muller, M. (2012) The HMG-Box Transcription Factor Sox4b Is Required for Pituitary Expression of gata2a and Specification of Thyrotrope and Gonadotrope Cells in Zebrafish. Molecular endocrinology (Baltimore, Md.). 26(6):1014-1027
Detry, B., Erpicum, C., Paupert, J., Blacher, S., Maillard, C., Bruyère, F., Pendeville, H., Remacle, T., Lambert, V., Balsat, C., Ormenese, S., Lamaye, F., Janssens, E., Moons, L., Cataldo, D., Kridelka, F., Carmeliet, P., Thiry, M., Foidart, J.M., Struman, I., and Noel, A. (2012) Matrix metalloproteinase-2 governs lymphatic vessel formation as an interstitial collagenase. Blood. 119(21):5048-5056
Arkhipova, V., Wendik, B., Devos, N., Ek, O., Peers, B., and Meyer, D. (2012) Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish. Developmental Biology. 365(1):290-302
Lambaerts, K., Van Dyck, S., Mortier, E., Ivarssonm, Y., Degeest, G., Luyten, A., Vermeiren, E., Peers, B., David, G., and Zimmermann, P. (2012) Syntenin, a syndecan adaptor and an Arf6 phosphatidylinositol 4,5-bisphosphate effector, is essential for epiboly and gastrulation cell movements in zebrafish. Journal of Cell Science. 125(5):1129-1140
Naye, F., Voz, M.L., Detry, N., Hammerschmidt, M., Peers, B., and Manfroid, I. (2012) Essential roles of zebrafish bmp2a, fgf10 and fgf24 in the specification of the ventral pancreas. Molecular biology of the cell. 23(5):945-954
Dalcq, J., Pasque, V., Ghaye, A., Larbuisson, A., Motte, P., Martial, J.A., and Muller, M. (2012) RUNX3, EGR1 and SOX9B Form a Regulatory Cascade Required to Modulate BMP-Signaling during Cranial Cartilage Development in Zebrafish. PLoS One. 7(11):e50140
Laudadio, I., Manfroid, I., Achouri, Y., Schmidt, D., Wilson, M.D., Cordi, S., Thorrez, L., Knoops, L., Jacquemin, P., Schuit, F., Pierreux, C.E., Odom, D.T., Peers, B., and Lemaigre, F.P. (2012) A Feedback Loop Between The Liver-Enriched Transcription Factor Network and Mir-122 Controls Hepatocyte Differentiation. Gastroenterology. 142(1):119-29
Voz, M.L., Coppieters, W., Manfroid, I., Baudhuin, A., Von Berg, V., Charlier, C., Meyer, D., Driever, W., Martial, J.A., and Peers, B. (2012) Fast Homozygosity Mapping and Identification of a Zebrafish ENU-Induced Mutation by Whole-Genome Sequencing. PLoS One. 7(4):e34671
Pruvot, B., Jacquel, A., Droin, N., Auberger, P., Bouscary, D., Tamburini, J., Muller, M., Fontenay, M., Chluba, J., and Solary, E. (2011) Leukemic cell xenograft in zebrafish embryo for investigating drug efficacy. Haematologica. 96(4):612-616
Verbruggen, V., Ek, O., Georlette, D., Delporte, F., Von Berg, V., Detry, N., Biemar, F., Coutinho, P., Martial, J.A., Voz, M.L., Manfroid, I., and Peers, B. (2010) The Pax6b homeodomain is dispensable for pancreatic endocrine cell differentiation in zebrafish. The Journal of biological chemistry. 285(18):13863-13873
Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L. (2010) Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells. Developmental Biology. 340(2):397-407
Muller, M., Dalcq, J., Aceto, J., Larbuisson, A., Pasque, V., Nourizadeh-Lilladadi, R., Alestrom, P., Martial, J. A. (2010) The function of the Egr1 transcription factor in cartilage formation and adaptation to microgravity in zebrafish, Danio rerio. Journal of Applied Ichthyology. 26(2):239-244
Soyer, J., Flasse, L., Raffelsberger, W., Beucher, A., Orvain, C., Peers, B., Ravassard, P., Vermot, J., Voz, M.L., Mellitzer, G., and Gradwohl, G. (2010) Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development. Development (Cambridge, England). 137(2):203-212
Toro, S., Wegner, J., Muller, M., Westerfield, M., and Varga, Z.M. (2009) Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis. Gene Expression Patterns. 9(4):200-208
Delporte, F.M., Pasque, V., Devos, N., Manfroid, I., Voz, M.L., Motte, P., Biemar, F., Martial, J.A., and Peers, B. (2008) Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors. BMC Developmental Biology. 8:53
Pendeville, H., Winandy, M., Manfroid, I., Nivelles, O., Motte, P., Pasque, V., Peers, B., Struman, I., Martial, J.A., and Voz, M.L. (2008) Zebrafish Sox7 and Sox18 function together to control arterial-venous identity. Developmental Biology. 317(2):405-416
Manfroid, I., Delporte, F., Baudhuin, A., Motte, P., Neumann, C.J., Voz, M.L., Martial, J.A., and Peers, B. (2007) Reciprocal endoderm-mesoderm interactions mediated by fgf24 and fgf10 govern pancreas development. Development (Cambridge, England). 134(22):4011-4021
Stetsyuk, V., Peers, B., Mavropoulos, A., Verbruggen, V., Thisse, B., Thisse, C., Motte, P., Duvillie, B., and Scharfmann, R. (2007) Calsenilin is required for endocrine pancreas development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 236(6):1517-1525
Peeters, H., Voz, M.L., Verschueren, K., De Cat, B., Pendeville, H., Thienpont, B., Schellens, A., Belmont, J.W., David, G., Van De Ven, W.J., Fryns, J.P., Gewillig, M., Huylebroeck, D., Peers, B., and Devriendt, K. (2006) Sesn1 is a Novel Gene for Left-Right Asymmetry and Mediating Nodal Signaling. Hum. Mol. Genet.. 15(22):3369-3377
Nichane, M., Van Campenhout, C., Pendeville, H., Voz, M.L., and Bellefroid, E.J. (2006) The Na(+)/PO(4) cotransporter SLC20A1 gene labels distinct restricted subdomains of the developing pronephros in Xenopus and zebrafish embryos. Gene Expression Patterns. 6(7):667-672
Van Campenhout, C., Nichane, M., Antoniou, A., Pendeville, H., Bronchain, O.J., Marine, J.C., Mazabraud, A., Voz, M.L., and Bellefroid, E.J. (2006) Evi1 is specifically expressed in the distal tubule and duct of the Xenopus pronephros and plays a role in its formation. Developmental Biology. 294(1):203-219
Pendeville, H., Peers, B., Kas, K., and Voz, M.L. (2006) Cloning and embryonic expression of zebrafish PLAG genes. Gene Expression Patterns. 6(3):267-276
Lopez, M., Nica, G., Motte, P., Martial, J.A., Hammerschmidt, M., and Muller, M. (2006) Expression of the somatolactin beta gene during zebrafish embryonic development. Gene Expression Patterns. 6(2):156-161
Mavropoulos, A., Devos, N., Biemar, F., Zecchin, E., Argenton, F., Edlund, H., Motte, P., Martial, J.A., and Peers, B. (2005) sox4b is a key player of pancreatic alpha cell differentiation in zebrafish. Developmental Biology. 285(1):211-223
de Cat, B., Muyldermans, S.Y., Coomans, C., Degeest, G., Vanderschueren, B., Creemers, J., Biemar, F., Peers, B., and David, G. (2003) Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. The Journal of cell biology. 163(3):625-635
Close, R., Toro, S., Martial, J., and Muller, M. (2002) Expression of the zinc finger Egr1 gene during zebrafish embryonic development. Mechanisms of Development. 118(1-2):269-272
Biemar, F., Baraldi, F., Devos, N., Holzschuh, J., Martial, J.A., Driever, W., and Peers, B. (2002) Analysis of meis genes expression in zebrafish suggests a role in the development of organs derived from the endoderm. Developmental Biology. 247(2):495
Devos, N., Deflorian, G., Biemar, F., Bortolussi, M., Martial, J.A., Peers, B., and Argenton, F. (2002) Differential expression of two somatostatin genes during zebrafish embryonic development. Mechanisms of Development. 115(1-2):133-137
Biemar, F., Devos, N., Martial, J.A., Driever, W., and Peers, B. (2001) Cloning and expression of the TALE superclass homeobox Meis2 gene during zebrafish embryonic development. Mechanisms of Development. 109(2):427-431
Molina, A., Iyengar, A., Marins, L.F., Biemar, F., Hanley, S., Maclean, N., Smith, T.J., Martial, J.A., and Muller, M. (2001) Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene. BBA Gene Structure and Expression. 1520(3):195-202
Molina, A., Di Martino, E., Martial, J.A., and Muller, M. (2001) Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element. The Biochemical journal. 356(2):353-359
Biemar, F., Argenton, F., Schmidtke, R., Epperlein, S., Peers, B., and Driever, W. (2001) Pancreas development in zebrafish: early dispersed appearance of endocrine hormone expressing cells and their convergence to form the definitive islet. Developmental Biology. 230(2):189-203
Molina, A., Biemar, F., Müller, F., Iyengar, A., Prunet, P., Maclean, N., Martial, J.A., and Muller, M. (2000) Cloning and expression analysis of an inducible HSP70 gene from tilapia fish. FEBS letters. 474(1):5-10