Gene
keap1b
- ID
- ZDB-GENE-080508-1
- Name
- kelch-like ECH-associated protein 1b
- Symbol
- keap1b Nomenclature History
- Previous Names
-
- klhl19 (1)
- zgc:171654
- Type
- protein_coding_gene
- Location
- Chr: 6 Mapping Details/Browsers
- Description
- Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Involved in protein ubiquitination and ubiquitin-dependent protein catabolic process. Acts upstream of or within cellular response to prostaglandin stimulus; cellular response to xenobiotic stimulus; and larval feeding behavior. Predicted to be located in inclusion body. Predicted to be part of Cul3-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. Is expressed in several structures, including gill; head; heart; liver; and pleuroperitoneal region. Human ortholog(s) of this gene implicated in gallbladder cancer. Orthologous to human KEAP1 (kelch like ECH associated protein 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 3 figures from 3 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- 1 Figure from Nguyen et al., 2020
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| it308 | Allele with one deletion | Exon 4 | Frameshift | CRISPR |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-keap1b | Ong et al., 2023 | |
| CRISPR2-keap1b | Ong et al., 2023 | |
| MO1-keap1b | N/A | Van Hall-Beauvais et al., 2022 |
| MO2-keap1b | N/A | Van Hall-Beauvais et al., 2022 |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | BTB/Kelch-associated | BTB-kelch protein | BTB/POZ domain | Kelch-like ECH-associated protein 1, BACK domain | Kelch-like ECH-associated protein 1, BTB/POZ domain | Kelch repeat type 1 | Kelch-type beta propeller | SKP1/BTB/POZ domain superfamily |
|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A2R8Q1W5 | InterPro | 587 | ||||||||
| UniProtKB:A0A8M9Q5W8 | InterPro | 593 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
keap1b-201
(1)
|
Ensembl | 3,053 nt | ||
| mRNA |
keap1b-202
(1)
|
Ensembl | 3,059 nt | ||
| mRNA |
keap1b-203
(1)
|
Ensembl | 2,911 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
No data available
| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | Fosmid | ZFOS-323E3 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:171654 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001113477 (1) | 2944 nt | ||
| Genomic | GenBank:CU693486 (1) | 42264 nt | ||
| Polypeptide | UniProtKB:A0A8M9Q5W8 (1) | 593 aa |
| Species | Symbol | Chromosome | Accession # | Evidence |
|---|---|---|---|---|
| Human | KEAP1 | 19 | Nucleotide sequence comparison (1) Amino acid sequence comparison (3) Conserved genome location (synteny) (1) Phylogenetic tree (2) | |
| Mouse | Keap1 | 9 | Phylogenetic tree (2) Conserved genome location (synteny) (1) Amino acid sequence comparison (2) Nucleotide sequence comparison (1) |
- Bian, L., Nguyen, V.T., Tamaoki, J., Endo, Y., Dong, G., Sato, A., Kobayashi, M. (2023) Genetic hyperactivation of Nrf2 causes larval lethality in Keap1a and Keap1b-double-knockout zebrafish. Redox Biology. 62:102673102673
- Ong, A.J.S., Bladen, C.E., Tigani, T.A., Karamalakis, A.P., Evason, K.J., Brown, K.K., Cox, A.G. (2023) The KEAP1-NRF2 pathway regulates TFEB/TFE3-dependent lysosomal biogenesis. Proceedings of the National Academy of Sciences of the United States of America. 120:e2217425120e2217425120
- Ravindra, J., Ug, Y., Pandyanda Nanjappa, D., Kalladka, K., Dhakal, R., Chakraborty, A., Chakraborty, G. (2023) Allicin extracted from Allium sativum shows potent anti-cancer and antioxidant properties in zebrafish. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 169:115854115854
- Yumimoto, K., Sugiyama, S., Motomura, S., Takahashi, D., Nakayama, K.I. (2023) Molecular evolution of Keap1 was essential for adaptation of vertebrates to terrestrial life. Science advances. 9:eadg2379
- Lasbleiz, C., Peyrel, A., Tarot, P., Sarniguet, J., Crouzier, L., Cubedo, N., Delprat, B., Rossel, M., Maurice, T., Liévens, J.C. (2022) Sigma-1 receptor agonist PRE-084 confers protection against TAR DNA-binding protein-43 toxicity through NRF2 signalling. Redox Biology. 58:102542102542
- Van Hall-Beauvais, A., Poganik, J.R., Huang, K.T., Parvez, S., Zhao, Y., Lin, H.Y., Liu, X., Long, M.J.C., Aye, Y. (2022) Z-REX uncovers a bifurcation in function of Keap1 paralogs. eLIFE. 11:
- Alifu, Y., Kofuji, S., Sunaga, S., Kusaba, M., Hirayama, J., Nishina, H. (2021) The Light-Inducible Genes Per2, Cry1a, and Cry2a Regulate Oxidative Status in Zebrafish. Biological & pharmaceutical bulletin. 44:1160-1165
- Li, E., Wang, Y., Li, Q., Li, L., Wei, L. (2021) Protective Effects of Sal B on Oxidative Stress-Induced Aging by Regulating the Keap1/Nrf2 Signaling Pathway in Zebrafish. Molecules. 26(17):
- Nguyen, V.T., Bian, L., Tamaoki, J., Otsubo, S., Muratani, M., Kawahara, A., Kobayashi, M. (2020) Generation and characterization of keap1a- and keap1b-knockout zebrafish. Redox Biology. 36:101667
- Shaw, P., Sen, A., Mondal, P., Dey Bhowmik, A., Rath, J., Chattopadhyay, A. (2020) Shinorine ameliorates chromium induced toxicity in zebrafish hepatocytes through the facultative activation of Nrf2-Keap1-ARE pathway. Aquatic toxicology (Amsterdam, Netherlands). 228:105622
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