FIGURE SUMMARY
Title

Using zebrafish to learn statistical analysis and mendelian genetics

Authors
Lindemann, S., Senkler, J., Auchter, E., and Liang, J.O.
Source
Full text @ Zebrafish
Fig. 1

Dihybrid cross with fish carrying the GloYFP transgene and gol mutation. The parental (P0) generation consisted of a female fish heterozygous for the GloYFP transgene and homozygous for the gol mutation and a WT male fish that was not carrying the transgene or the gol mutation. Genotypes at the different loci are separated by semicolons with the genotype at the transgene locus listed first. The F1 generation was produced through a single mating of these adult fish, with one progeny of each phenotype shown. Images are lateral views, anterior to the left and dorsal to the top. Images of all of the progeny are included in Supplementary Figure 1. WT, wild type.
Fig. 3

Dihybrid cross of parents heterozygous for GloYFP and gol. The parental (P0) generation consisted of a female and a male fish heterozygous for the GloYFP transgene and the gol mutation. Genotypes at the different loci are separated by semicolons with the genotype at the transgene locus listed first. The F1 generation was produced through a single mating of the P0 pair, with one progeny of each phenotype shown. Images are lateral views, anterior to the left and dorsal to the top. Images of all of the progeny are included in Supplementary Figure 2.
Fig. 5

Trihybrid cross with fish carrying Glo transgenes and the gol mutation. The parental (P0) generation consisted of a female fish heterozygous for the GloRFP transgene and heterozygous for the gol mutation and a male fish heterozygous for the GloYFP transgene and heterozygous for the gol mutation. Genotypes at the different loci are separated by semicolons with the genotype at the GloYFP transgene locus listed first. The F1 generation was produced through a single mating of the P0 pair, with one progeny of each phenotype shown. Images are lateral views, anterior to the left and dorsal to the top. Images of all of the progeny are included in Supplementary Figure 3.
Fig. 7

Trihybrid cross with fish carrying Glo transgenes and the lof mutation. The parental (P0) generation consisted of a female not carrying a transgene and heterozygous for the lof mutation and a male fish heterozygous for the GloRFP and GloYFP transgenes. Genotypes at the different loci are separated by semicolons, the GloYFP locus listed first. The F1 generation was produced through a single mating of the P0 pair, with one progeny of each phenotype shown. Images are lateral views, anterior to the left and dorsal to the top. Images of all of the progeny are included in Supplementary Figure 4.
Fig. 9

Cross of fish carrying the cyc mutation. The parental (P0) generation consisted of a female and a male fish heterozygous for the cyc mutation. A single natural mating of these fish produced progeny, the F1 generation, with two normal eyes or a single cyclopic eye. The fish with cyclopic eyes also had a severe ventral curvature in the anterior– posterior axis. A typical embryo at 3 days postfertilization is shown for each phenotype. All images are lateral views with anterior to the left and dorsal to the top. Images of all progeny from this cross are included in Supplementary Figure 5.
Fig. 11

Cross of fish carrying the sqt mutation. The parental (P0) generation consisted of a female and male fish that were heterozygous for the sqt mutation. As with the cyc cross, a single natural mating of sqt heterozygotes fish produced progeny, the F1 generation, with two normal eyes or a single cyclopic eye. Adults are shown in lateral views, anterior to the left, embryos in ventral views with anterior to the left. Pictures of all of the progeny from this cross are included in Supplementary Figure 6.
Fig. 14

GloFish fluorescence. (A) Yellow GloFish under white light. (B) Yellow GloFish under blue light/GFP filter. (C) Head of yellow GloFish under blue light/ GFP filter. (D) Red GloFish under white light. (E) Red GloFish under green light/ RFP filter. (F) Head of red GloFish under green light/RFP filter. (G) Orange Glo- Fish under white light. (H) Orange Glo- Fish under blue light/GFP filter. (I) Orange GloFish under green light/RFP filter. Images are lateral views, anterior to the left and dorsal to the top. GFP, green fluorescent protein; RFP, red fluorescent protein.
Acknowledgments
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