PUBLICATION
An early developmental role for Eph-ephrin interaction during vertebrate gastrulation
- Authors
- Oates, A.C., Lackmann, M., Power, M.A., Brennan, C., Smith, F.M., Do, C., Evans, B., Holder, N., and Boyd, A.W.
- ID
- ZDB-PUB-990628-7
- Date
- 1999
- Source
- Mechanisms of Development 83(1-2): 77-94 (Journal)
- Registered Authors
- Boyd, Andrew W., Do, Cuong, Holder, Nigel, Oates, Andrew
- Keywords
- vertebrate embryogenesis; development; Eph, EphA3, EphA1, EphB1; ephrin; ephrin-A5; L1; HEK; LERK-7; gastrulation; somitogenesis; CNS patterning; mesoderm; boundary formation; zebrafish; Danio rerio; RNA microinjection; BIAcore analysis; Fc-fusion; whole-mount; in situ hybridization
- MeSH Terms
-
- Animals
- Cell Movement
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/anatomy & histology
- Ephrin-A1
- Ephrin-A3
- Ephrin-A5
- Ephrin-B1
- Gastrula/metabolism*
- Gene Expression Regulation, Developmental
- Genes, Dominant
- Humans
- Kinetics
- Membrane Proteins/analysis
- Membrane Proteins/metabolism*
- Multigene Family/physiology*
- Proteins/metabolism*
- RNA, Messenger/pharmacology
- Time Factors
- Zebrafish/embryology
- PubMed
- 10381569 Full text @ Mech. Dev.
Citation
Oates, A.C., Lackmann, M., Power, M.A., Brennan, C., Smith, F.M., Do, C., Evans, B., Holder, N., and Boyd, A.W. (1999) An early developmental role for Eph-ephrin interaction during vertebrate gastrulation. Mechanisms of Development. 83(1-2):77-94.
Abstract
Eph receptor tyrosine kinases (RTK) and their ephrin ligands are involved in the transmission of signals which regulate cytoskeletal organisation and cell migration, and are expressed in spatially restricted patterns at discrete phases during embryogenesis. Loss of function mutants of Eph RTK or ephrin genes result in defects in neuronal pathfinding or cell migration. In this report we show that soluble forms of human EphA3 and ephrin-A5, acting as dominant negative inhibitors, interfere with early events in zebrafish embryogenesis. Exogenous expression of both proteins results in dose-dependent defects in somite development and organisation of the midbrain-hindbrain boundary and hindbrain. The nature of the defects as well as the distribution and timing of expression of endogenous ligands/receptors for both proteins suggest that Eph-ephrin interaction is required for the organisation of embryonic structures by coordinating the cellular movements of convergence during gastrulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping