PUBLICATION
Origin of gene overlap. The case of tcp1 and acat2
- Authors
- Shintani, S., O'hUigin, C., Toyosawa, S., Michalova, V., and Klein, J.
- ID
- ZDB-PUB-990628-24
- Date
- 1999
- Source
- Genetics 152(2): 743-754 (Journal)
- Registered Authors
- Klein, Jan, O'hUigin, Colm
- Keywords
- none
- MeSH Terms
-
- Acetyl-CoA C-Acetyltransferase/genetics
- Alligators and Crocodiles
- Amino Acid Sequence
- Animals
- Base Sequence
- Chaperonin Containing TCP-1
- Chaperonins/genetics
- Cloning, Molecular
- DNA/chemistry
- DNA/genetics
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Evolution, Molecular*
- Exons
- Genes/genetics
- Genes, Overlapping/genetics*
- Humans
- Introns
- Macropodidae
- Mice
- Molecular Sequence Data
- Opossums
- Platypus
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Xenopus laevis
- Zebrafish
- PubMed
- 10353914 Full text @ Genetics
Citation
Shintani, S., O'hUigin, C., Toyosawa, S., Michalova, V., and Klein, J. (1999) Origin of gene overlap. The case of tcp1 and acat2. Genetics. 152(2):743-754.
Abstract
The human acetyl-CoA acetyltransferase 2 gene, ACAT2, codes for a thiolase, an enzyme involved in lipid metabolism. The human T-complex protein 1 gene, TCP1, encodes a molecular chaperone of the chaperonin family. The two genes overlap by their 3'-untranslated regions, their coding sequences being located on opposite DNA strands in a tail-to-tail orientation. To find out how the overlap might have arisen in evolution, the homologous genes of the zebrafish, the African toad, caiman, platypus, opossum, and wallaby were identified. In each species, standard or long polymerase chain reactions were used to determine whether the ACAT2 and TCP1 homologs are closely linked and, if so, whether they overlap. The results reveal that the overlap apparently arose during the transition from therapsid reptiles to mammals and has been retained for >200 million years. Part of the overlapping untranslated region shows remarkable sequence conservation. The overlap presumably arose during the chromosomal rearrangement that brought the two unrelated and previously separated genes together. One or both of the transposed genes found by chance signals that are necessary for the processing of their transcripts to be present on the noncoding strand of the partner gene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping