ZFIN ID: ZDB-PUB-990427-14
Use of the Gal4-UAS technique for targeted gene expression in the zebrafish
Scheer, N. and Campos-Ortega, J.A.
Date: 1999
Source: Mechanisms of Development   80(2): 153-158 (Journal)
Registered Authors: Campos-Ortega, Jose, Scheer, Nico
Keywords: zebrafish; GAL4-UAS system; transgenic lines
MeSH Terms:
  • Actins/genetics
  • Adenovirus E1B Proteins/genetics
  • Animals
  • Animals, Genetically Modified
  • Cloning, Molecular
  • Crosses, Genetic
  • DNA-Binding Proteins
  • Embryo, Nonmammalian/metabolism
  • Enhancer Elements, Genetic
  • Fungal Proteins/genetics*
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter*
  • Genes, myc
  • In Situ Hybridization
  • Membrane Proteins/biosynthesis
  • Membrane Proteins/genetics
  • Pilot Projects
  • Promoter Regions, Genetic*
  • Proto-Oncogene Proteins c-myc/biosynthesis
  • Receptor, Notch1
  • Receptors, Cell Surface*
  • Recombinant Fusion Proteins/biosynthesis
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/physiology
  • Regulatory Sequences, Nucleic Acid*
  • Saccharomyces cerevisiae Proteins*
  • Simian virus 40/genetics
  • Simplexvirus/enzymology
  • Simplexvirus/genetics
  • Thymidine Kinase/genetics
  • Transcription Factors/genetics*
  • Transcription, Genetic*
  • Transgenes
  • Viral Proteins/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 10072782 Full text @ Mech. Dev.
ABSTRACT
The most common way to analyze the function of cloned genes in zebrafish is to misexpress the gene product or an altered variant of it by mRNA injection. However, mRNA injection has several disadvantages. The GAL4-UAS system for targeted gene expression allows one to overcome some of these disadvantages. To test the GAL4-UAS system in zebrafish, we generated two different kinds of stable transgenic lines, carrying activator and effector constructs, respectively. In the activator lines the gene for the yeast transcriptional activator GAL4 is under the control of a given promoter, while in the effectors the gene of interest is fused to the sequence of the DNA-binding motif of GAL4 (UAS). Crosses of animals from the activator and effector lines show that effector genes are transcribed with the spatial pattern of the activators. This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes.
ADDITIONAL INFORMATION