PUBLICATION
Use of the Gal4-UAS technique for targeted gene expression in the zebrafish
- Authors
- Scheer, N. and Campos-Ortega, J.A.
- ID
- ZDB-PUB-990427-14
- Date
- 1999
- Source
- Mechanisms of Development 80(2): 153-158 (Journal)
- Registered Authors
- Campos-Ortega, Jose, Scheer, Nico
- Keywords
- zebrafish; GAL4-UAS system; transgenic lines
- MeSH Terms
-
- Actins/genetics
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- Viral Proteins/genetics
- Zebrafish/embryology
- Zebrafish/genetics*
- Saccharomyces cerevisiae Proteins*
- Thymidine Kinase/genetics
- Transcription, Genetic*
- Receptors, Cell Surface*
- In Situ Hybridization
- Transgenes
- Regulatory Sequences, Nucleic Acid*
- Pilot Projects
- Animals, Genetically Modified
- Promoter Regions, Genetic*
- Crosses, Genetic
- Simian virus 40/genetics
- Receptor, Notch1
- Adenovirus E1B Proteins/genetics
- Fungal Proteins/genetics*
- Embryo, Nonmammalian/metabolism
- Genes, myc
- Recombinant Fusion Proteins/biosynthesis
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/physiology
- Proto-Oncogene Proteins c-myc/biosynthesis
- Animals
- Simplexvirus/enzymology
- Simplexvirus/genetics
- Gene Expression Regulation, Developmental*
- Membrane Proteins/biosynthesis
- Membrane Proteins/genetics
- Transcription Factors/genetics*
- Genes, Reporter*
- Cloning, Molecular
- PubMed
- 10072782 Full text @ Mech. Dev.
Citation
Scheer, N. and Campos-Ortega, J.A. (1999) Use of the Gal4-UAS technique for targeted gene expression in the zebrafish. Mechanisms of Development. 80(2):153-158.
Abstract
The most common way to analyze the function of cloned genes in zebrafish is to misexpress the gene product or an altered variant of it by mRNA injection. However, mRNA injection has several disadvantages. The GAL4-UAS system for targeted gene expression allows one to overcome some of these disadvantages. To test the GAL4-UAS system in zebrafish, we generated two different kinds of stable transgenic lines, carrying activator and effector constructs, respectively. In the activator lines the gene for the yeast transcriptional activator GAL4 is under the control of a given promoter, while in the effectors the gene of interest is fused to the sequence of the DNA-binding motif of GAL4 (UAS). Crosses of animals from the activator and effector lines show that effector genes are transcribed with the spatial pattern of the activators. This work smoothes the way for a novel method of misexpression of gene products in zebrafish in order to analyze the function of genes in developmental processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping