ZFIN ID: ZDB-PUB-990106-18
Isolation, expression and regulation of a zebrafish paraxis homologue
Shanmugalingam, S. and Wilson, S.W.
Date: 1998
Source: Mechanisms of Development   78: 85-89 (Journal)
Registered Authors: Shanmugalingam, Shantha, Wilson, Steve
Keywords: somite; spadetail; cyclops; notail; one-eyed-pinhead
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins/biosynthesis
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/isolation & purification
  • Gastrula/metabolism
  • Helix-Loop-Helix Motifs/genetics*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Homeodomain Proteins/physiology
  • Humans
  • Mesoderm/metabolism
  • Molecular Sequence Data
  • Morphogenesis/genetics
  • Nuclear Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Somites/metabolism*
  • Species Specificity
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription Factors/physiology
  • Vertebrates/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 9858695 Full text @ Mech. Dev.
The formation of somites involves the subdivision of segmented presomitic mesoderm into segmentally arranged somite blocks. In mice and chicks, the basic-helix-loop-helix (bHLH) gene, paraxis, is involved in this process. Here, we report the isolation of a zebrafish homologue of paraxis, par1. par1 is expressed in presomitic paraxial mesoderm from late gastrula stages, and expression is maintained in ventrolateral cells after somite formation. In spt- embryos, par1 expression is both delayed and severely reduced whereas in flh- embryos, ectopic transcripts are detected in axial mesoderm. Spatial regulation of par1 expression within the somites is affected in several mutants with defects in axial midline tissues.