PUBLICATION
Evidence for a fizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation
- Authors
- Nasevicius, A., Hyatt, T., Kim, H., Guttman, J., Walsh, E., Sumanas, S., Wang, Y.S., and Ekker, S.C.
- ID
- ZDB-PUB-981215-13
- Date
- 1998
- Source
- Development (Cambridge, England) 125: 4283-4292 (Journal)
- Registered Authors
- Ekker, Stephen C., Hyatt, Tana, Kim, Hyon, Nasevicius, Aidas, Sumanas, Saulius, Walsh, Emily, Wang, Yihong
- Keywords
- zebrafish; frizzled; wnt signalling; dorsal organizer; mesoderm dorsoventral patterning
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Body Patterning*
- Calcium-Calmodulin-Dependent Protein Kinases/metabolism
- Calcium-Calmodulin-Dependent Protein Kinases/physiology
- Cytoskeletal Proteins
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiology
- Gene Expression Regulation, Developmental*
- Glycogen Synthase Kinase 3
- In Situ Hybridization
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Membrane Proteins/physiology*
- Mesoderm/metabolism
- Mesoderm/physiology*
- Molecular Sequence Data
- Mutagenesis
- Proteins/physiology
- Wnt Proteins
- Zebrafish/embryology
- Zebrafish Proteins*
- PubMed
- 9753682 Full text @ Development
Citation
Nasevicius, A., Hyatt, T., Kim, H., Guttman, J., Walsh, E., Sumanas, S., Wang, Y.S., and Ekker, S.C. (1998) Evidence for a fizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation. Development (Cambridge, England). 125:4283-4292.
Abstract
We have used zebrafish as a model system for the study of vertebrate dorsoventral patterning. We isolated a maternally expressed and dorsal organizer localized member of the frizzled family of wnt receptors. Wild-type and dominant, loss-of-function molecules in misexpression studies demonstrate frizzled function is necessary and sufficient for dorsal mesoderm specification. frizzled activity is antagonized by the action of GSK-3, and we show GSK-3 is also required for zebrafish dorsal mesoderm formation. frizzled cooperatively interacts with the maternally encoded zebrafish wnt8 protein in dorsal mesodermal fate determination. This frizzled -mediated wnt pathway for dorsal mesoderm specification provides the first evidence for the requirement of a wnt-like signal in vertebrate axis determination.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping