Role of semaphorins in growth cone guidance in the zebrafish embryo
- Kuwada, J.Y.
- Neuroscience research 21 sup. 1: 4 (Abstract)
- Registered Authors
- Kuwada, John
- MeSH Terms
- none Full text @ Neurosci. Res.
Kuwada, J.Y. (1997) Role of semaphorins in growth cone guidance in the zebrafish embryo. Neuroscience research. 21 sup. 1:4.
Growth cones are guided to their targets by a variety of mechanisms including repulsive ones. Among molecules that can repel growth cones are members of the Semaphorin/Collapsin family. In order to examine how Semaphorins affect the behavior of growth cones in developing embryos, we cloned and analyzed sema Z1, a homolog to sema III/D/collapsin-1, in the zebrafish embryo. The expression pattern of sema Z1 suggests that it may influence specific growth cones. In support of this, we found that application of recombinant Sema Z1 collapses growth cones both in vitro and in vivo. Furthermore, sema Z1 is aberrantly expressed in the floating head mutant, and growth cones responsive to Sema Z1 extend aberrantly in accord with the change in expression. Finally, time lapse analysis of growth cones that are responsive to Sema Z1 shows that they collapse and retract during normal outgrowth. This suggests that Sema Z1 may normally play an important role in guiding specific growth cones.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes