PUBLICATION
Restricted expression of the homeobox gene prox 1 in developing zebrafish
- Authors
- Glasgow, E. and Tomarev, S.I.
- ID
- ZDB-PUB-981105-4
- Date
- 1998
- Source
- Mechanisms of Development 76: 175-178 (Journal)
- Registered Authors
- Glasgow, Eric
- Keywords
- transcription factor; prospero; eye; lens; hindbrain; spinal cord; lateral line; trigeminal ganglia; muscle; otic vesicle; homeobox; development; zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cross Reactions
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental*
- Homeodomain Proteins/genetics*
- Homeodomain Proteins/immunology
- Homeodomain Proteins/metabolism
- Humans
- Immunohistochemistry
- Mice
- Molecular Sequence Data
- Tumor Suppressor Proteins
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 9767161 Full text @ Mech. Dev.
Citation
Glasgow, E. and Tomarev, S.I. (1998) Restricted expression of the homeobox gene prox 1 in developing zebrafish. Mechanisms of Development. 76:175-178.
Abstract
Prox 1 is a vertebrate homeobox gene which is homologous to the Drosophila transcription factor, prospero. We have isolated a prox 1 cDNA from zebrafish, which encodes a protein that has 82%, 84% and 83% amino acid identity with chicken, mouse and human Prox 1, respectively. Antibodies raised against human Prox 1 cross-react with zebrafish Prox 1 and are used here to determine the expression patterns of Prox 1 during zebrafish embryogenesis by whole-mount immunohistochemistry. In the 10-somite embryo, Prox 1 is expressed over the prospective lens placode and over a broad region of epithelium extending from the eye to the otic vesicle. As embryogenesis proceeds, Prox 1 expression in the eye lens becomes intense, and is detected in maturing muscle pioneer cells and superficial muscle cells. In the CNS, Prox 1 is expressed in a stripe along the forebrain-midbrain boundary, in a segmented pattern in the ventral hindbrain, and in selected cells of the ventral spinal cord. Additional sites of Prox 1 expression include the lateral line primordium, the trigeminal ganglia, the otic vesicle and occasional endodermal cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping