PUBLICATION
Physiological properties of the Mauthner system in the adult zebrafish
- Authors
- Hatta, K. and Korn, H.
- ID
- ZDB-PUB-980602-8
- Date
- 1998
- Source
- The Journal of comparative neurology 395: 493-509 (Journal)
- Registered Authors
- Hatta, Kohei, Korn, Henri
- Keywords
- axon cap; field effects; electrical inhibition; chemical inhibition; auditory response; electrical excitation
- MeSH Terms
-
- Animals
- Axons/physiology
- Axons/ultrastructure
- Brain/physiology
- Electric Conductivity
- Goldfish/physiology
- In Vitro Techniques
- Interneurons/physiology
- Membrane Potentials
- Neurons/cytology*
- Neurons/physiology*
- Neurons/ultrastructure
- Reaction Time
- Spinal Cord/cytology
- Spinal Cord/physiology*
- Synapses/physiology
- Synaptic Transmission
- Zebrafish/physiology*
- PubMed
- 9619502 Full text @ J. Comp. Neurol.
Citation
Hatta, K. and Korn, H. (1998) Physiological properties of the Mauthner system in the adult zebrafish. The Journal of comparative neurology. 395:493-509.
Abstract
We investigated the morphological and electrophysiological properties of the Mauthner (M-) cell and its networks in the adult zebrafish (Danio rerio) in comparison with those in the goldfish (Carassius auratus). The zebrafish M-cell has an axon cap, a high resistivity structure which surrounds the initial segment of the M-axon, and accounts for an unusual amplification of the fields generated within and around it. Second, extra- and intracellular recordings were performed with microelectrodes. The resting potential was approximately -80 mV with an input resistance of approximately 0.42 M omega. The M-cell extracellular field was large (10-20 mV), close to the axon hillock, and the latency of antidromic spikes short (approximately 0.4 milliseconds), confirming a high conduction velocity in the M-axon. The extrinsic hyperpolarizing potential (EHP), which signals firing of presynaptic cells and collateral inhibition, was markedly lower at frequencies of spinal stimulation > approximately 5/second, suggesting an organization of the recurrent collateral network similar to that in the goldfish. Inhibitory postsynaptic potentials (IPSPs) were highly voltage-dependent; their decay time constant was increased by depolarizations. The presynaptic neurons which are numerous could be identified by their passive hyperpolarizing potential (PHP) produced by the M-spike current. Auditory responses, mediated via mixed synapses (electrical and chemical), had short delays and hence are well suited to trigger the escape reaction. The similarities of their properties indicate that the wealth of information generated over decades in the goldfish can be extrapolated to the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping