zALK-8, a novel type I serine/threonine kinase receptor, is expressed throughout early zebrafish development
- Yelick, P.C., Abduljabbar, T.S., and Stashenko, P.
- Developmental Dynamics : an official publication of the American Association of Anatomists 211: 352-361 (Journal)
- Registered Authors
- Abduljabbar, Tariq, Yelick, Pamela C.
- novel serine/threonine kinase receptor; activin receptor-like-kinases; zebrafish development; transforming growth factor P receptor; family members
- MeSH Terms
- Activin Receptors
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- Embryo, Nonmammalian/metabolism*
- In Situ Hybridization
- Molecular Sequence Data
- Protein Serine-Threonine Kinases/genetics*
- Protein Serine-Threonine Kinases/metabolism*
- RNA, Messenger/analysis
- Receptors, Transforming Growth Factor beta/genetics
- Receptors, Transforming Growth Factor beta/metabolism
- Sequence Homology, Amino Acid
- 9566954 Full text @ Dev. Dyn.
Yelick, P.C., Abduljabbar, T.S., and Stashenko, P. (1998) zALK-8, a novel type I serine/threonine kinase receptor, is expressed throughout early zebrafish development. Developmental Dynamics : an official publication of the American Association of Anatomists. 211:352-361.
Here, we report the isolation and characterization of zebrafish activin receptor-like kinase-8 (zALK-8), a novel type I serine/threonine (ser/thr) kinase receptor of the transforming growth factor beta (TGF-beta) family. zALK-8 is novel, in that it contains an extracellular domain that is quite distinct from that of previously identified ALK receptors 1 through 7. Analysis of the predicted amino acid sequence of the 506 amino acid zALK-8 receptor reveals an ser/thr kinase domain characteristic of type I TGF-beta family member receptors. zALK-8, therefore, is a traditional type I ser/thr kinase receptor of the TGF-beta family, but it may exhibit novel ligand-binding activities. The developmental expression of zALK-8 mRNA was examined by wholemount in situ hybridization analysis using a probe from the 3'-untranslated sequence of zALK-8, which does not cross react with other members of the highly conserved TGF-beta receptor family. zALK-8 mRNA is present as a maternal message that is expressed ubiquitously before the start of zygotic transcription. By 16 hr postfertilization (hpf), zALK-8 mRNA is still expressed fairly evenly throughout the embryo. In 24-hpf embryos, zALK-8 mRNA is expressed predominantly in the developing eye and neural structures. By 48 hpf, zALK-8 mRNA is faintly detectable as a diffuse signal throughout the head. zALK-8 mRNA is not detectable by this method in 72-hpf or 96-hpf embryos. Northern analysis of zALK-8 mRNA in poly(A+) mRNA isolated from 6-9 hpf embryos detects a major transcript of 3.6 kb and a minor transcript of 4.3 kb. zALK-8 mRNA expression correlates well with known functions of TGF-beta family members as early axial patterning and mesoderm-inducing growth factors and as potent growth and differentiation factors in craniofacial development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes