|ZFIN ID: ZDB-PUB-980415-8|
Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis
Toyama, R., Kobayashi, M., Tomita, T., and Dawid, I.B.
|Source:||Mechanisms of Development 71: 197-200 (Journal)|
|Registered Authors:||Dawid, Igor B., Kobayashi, Makoto, Toyama, Reiko|
|Keywords:||zebrafish; LIM domain binding protein; protein-protein interaction|
|PubMed:||9507128 Full text @ Mech. Dev.|
Toyama, R., Kobayashi, M., Tomita, T., and Dawid, I.B. (1998) Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis. Mechanisms of Development. 71:197-200.
ABSTRACTLIM homeodomain proteins are developmental regulators whose functions depend on synergism with LIM domain binding proteins (Ldb proteins). We have isolated four members of the ldb gene family from the zebrafish, Danio rerio. Ldb1, Ldb2 and Ldb3 share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively. In overlay assays, Ldb proteins bind LIM homeodomain proteins and LMO1, but not zyxin or MLP. Whole mount in situ hybridization showed that zebrafish ldb1 is expressed ubiquitously from gastrulation onward. Ldb2 is ubiquitous at gastrulation, and later is found in many but not all tissues, especially the anterior central nervous system (CNS) and vasculature. Ldb3 mRNA was expressed primarily in the anterior CNS.