ZFIN ID: ZDB-PUB-980415-8
Expression of LIM-domain binding protein (ldb) genes during zebrafish embryogenesis
Toyama, R., Kobayashi, M., Tomita, T., and Dawid, I.B.
Date: 1998
Source: Mechanisms of Development   71: 197-200 (Journal)
Registered Authors: Dawid, Igor B., Kobayashi, Makoto, Toyama, Reiko
Keywords: zebrafish; LIM domain binding protein; protein-protein interaction
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins/biosynthesis
  • Carrier Proteins/genetics*
  • DNA-Binding Proteins/biosynthesis
  • DNA-Binding Proteins/genetics*
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics*
  • LIM Domain Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Zebrafish
  • Zebrafish Proteins*
PubMed: 9507128 Full text @ Mech. Dev.
LIM homeodomain proteins are developmental regulators whose functions depend on synergism with LIM domain binding proteins (Ldb proteins). We have isolated four members of the ldb gene family from the zebrafish, Danio rerio. Ldb1, Ldb2 and Ldb3 share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively. In overlay assays, Ldb proteins bind LIM homeodomain proteins and LMO1, but not zyxin or MLP. Whole mount in situ hybridization showed that zebrafish ldb1 is expressed ubiquitously from gastrulation onward. Ldb2 is ubiquitous at gastrulation, and later is found in many but not all tissues, especially the anterior central nervous system (CNS) and vasculature. Ldb3 mRNA was expressed primarily in the anterior CNS.