PUBLICATION
Cross-interactions between two members of the Dlx family of homeobox-containing genes during zebrafish development
- Authors
- Zerucha, T., Muller, J.-P., Chartrand, N., and Ekker, M.
- ID
- ZDB-PUB-980408-9
- Date
- 1997
- Source
- Biochemistry and cell biology = Biochimie et biologie cellulaire 75: 613-622 (Journal)
- Registered Authors
- Chartrand, Nathalie, Ekker, Marc, Zerucha, Ted
- Keywords
- Danio rerio; branchial arches; inner ear; homeodomain; transgenic animals
- MeSH Terms
-
- Animals
- Branchial Region/chemistry
- Branchial Region/embryology
- Gene Expression Regulation, Developmental/genetics*
- Genes, Homeobox/genetics*
- Homeodomain Proteins/genetics*
- Mice
- Microinjections
- Models, Genetic
- Neoplastic Stem Cells
- RNA, Messenger/analysis
- Recombinant Fusion Proteins
- Sequence Deletion
- Transcription Factors/genetics*
- Transcriptional Activation
- Transfection
- Tumor Cells, Cultured
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 9551183 Full text @ Biochem. Cell Biol.
Citation
Zerucha, T., Muller, J.-P., Chartrand, N., and Ekker, M. (1997) Cross-interactions between two members of the Dlx family of homeobox-containing genes during zebrafish development. Biochemistry and cell biology = Biochimie et biologie cellulaire. 75:613-622.
Abstract
The Dlx homeobox genes of vertebrates are transcribed in multiple cells of the embryo with overlapping patterns but often with different onsets of expression. Here we describe the interaction between two dlx genes, dlx3 and dlx4, during zebrafish development. The observation that dlx3 expression precedes that of dlx4 in the otic vesicle led us to investigate whether dlx3 had the ability to control expression of dlx4. Truncated versions of dlx3 were overexpressed in zebrafish embryos and the expression patterns of dlx4 were examined later in development. Overexpression of truncated forms of Dlx3 or of a Dlx3-Dlx2 chimera was found to result in perturbations in dlx4 expression. In addition, cotransfection experiments indicated the ability of Dlx3 to activate transcription through a 1.7-kb fragment of the 5 prime flanking region of dlx4. These results suggest that dlx4 is one of the target genes of dlx3 in embryos and that cross-regulatory interactions between Dlx genes may be one of the mechanisms responsible for their overlapping expression.
Les gènes à boite homéo de la famille Dlx des vertébrés sont coexprimés dans de nombreuses cellules de l'embryon. Nous rapportons l'interaction entre deux gènes dlx, dlx3 et dlx4, du danio (poisson-zèbre). L'expression de dlx3 précède celle de dlx4 dans les vésicules otiques au cours de leur développement, ce qui nous a amené à déterminer si dlx4 pouvait constituer un des gènes cibles de dlx3. Nous avons surexprimé des ARN messagers codant des versions tronquées de Dlx3 dans des embryons de danio et avons mesuré l'expression de dlx4 à des stades ultérieurs de développement. La surexpression des versions tronquées de Dlx3 ou d'une chimère Dlx3-Dlx2 causèrent des perturbations de l'expression de dlx4. De plus, des expériences de cotransfection ont démontré la capacité de Dlx3 d'activer la transcription d'un gène témoin contenant 1,7 kb de la séquence flanquante 5 prime de dlx4. Ces résultats suggèrent que dlx3 est un des activateurs de dlx4 chez l'embryon et que des interactions croisées entre les gènes Dlx sont, du moins en partie, responsables de leur coexpression au cours du développement.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping