ZFIN ID: ZDB-PUB-980123-3
The zebrafish thyroid hormone receptor alpha1 is expressed during early embryogenesis and can function in transcriptional repression
Essner, J.J., Breuer, J.J., Essner, R.D., Fahrenkrug, S.C., and Hackett, P.B., Jr.
Date: 1997
Source: Differentiation; research in biological diversity   62: 107-117 (Journal)
Registered Authors: Essner, Jeffrey, Fahrenkrug, Scott C., Hackett, Perry B.
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blastocyst/metabolism
  • Blotting, Northern
  • Cloning, Molecular
  • Embryo, Nonmammalian/metabolism*
  • Gastrula/metabolism
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter
  • Molecular Sequence Data
  • Oogenesis/physiology
  • RNA, Messenger/analysis
  • Receptors, Retinoic Acid/metabolism
  • Receptors, Thyroid Hormone/genetics*
  • Receptors, Thyroid Hormone/physiology
  • Repressor Proteins/genetics*
  • Repressor Proteins/physiology
  • Ribonucleases/metabolism
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*
  • Tretinoin/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 9447705 Full text @ Differentiation
ABSTRACT
Nuclear receptors are a large family of ligand dependent transcription factors which participate in many diverse processes during development. In this report, we describe the cloning of the zebrafish thyroid hormone receptor alpha 1 (TR alpha 1) gene, the cellular counterpart of the viral oncogene v-erbA. TR alpha 1 is expressed during oogenesis and maternally supplied to the embryo. TR alpha 1 is expressed again after the mid blastula transition. By examining the effects of increased expression of TR alpha 1 on expression of a reporter gene which responds to both TR alpha 1 and retinoic acid receptors (RARs), we show that the zebrafish TR alpha 1 can act as a repressor during early zebrafish development before thyroid hormone is present in the embryo. In addition, our data suggest that TR alpha 1 can repress retinoic acid (RA)-signaling during early development. We propose that TR alpha 1 functions during early development as a transcriptional repressor, similar to the constitutive repressor activity of its viral counterpart v-erbA, which regulates anterior-posterior (A/P) patterning by repressing RA-signaling.
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