PUBLICATION
Expression of c-ret in the zebrafish embryo: potential roles in motoneuronal development
- Authors
- Bisgrove, B.W., Raible, D.W., Walter, V., Eisen, J.S., and Grunwald, D.J.
- ID
- ZDB-PUB-971203-6
- Date
- 1997
- Source
- Journal of neurobiology 33(6): 749-768 (Journal)
- Registered Authors
- Bisgrove, Brent, Eisen, Judith S., Grunwald, David, Raible, David
- Keywords
- c-ret; receptor tyrosine kinase; zebrafish; embryogenesis; motoneurons
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Brain/physiology
- Branchial Region/embryology
- Branchial Region/physiology
- DNA/isolation & purification
- Drosophila Proteins*
- Embryo, Nonmammalian/physiology
- Enteric Nervous System/embryology
- Enteric Nervous System/physiology
- Gene Expression Regulation, Developmental*
- Immunohistochemistry
- Molecular Sequence Data
- Motor Neurons/physiology*
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/physiology*
- Proto-Oncogene Proteins c-ret
- RNA/isolation & purification
- Receptor Protein-Tyrosine Kinases/genetics
- Receptor Protein-Tyrosine Kinases/physiology*
- Sequence Analysis, DNA
- Spinal Cord/embryology
- Spinal Cord/physiology
- Zebrafish
- Zebrafish Proteins
- PubMed
- 9369149 Full text @ J. Neurobiol.
Citation
Bisgrove, B.W., Raible, D.W., Walter, V., Eisen, J.S., and Grunwald, D.J. (1997) Expression of c-ret in the zebrafish embryo: potential roles in motoneuronal development. Journal of neurobiology. 33(6):749-768.
Abstract
We have isolated and characterized the zebrafish ortholog of c-ret, a gene essential for renal organogenesis and enteric nervous system development in mammals. During zebrafish embryogenesis c-ret transcripts are expressed in a number of tissues including spinal motoneurons, pronephric ducts, cranial ganglia, pharyngeal arches, and the enteric nervous system. We have examined in detail the expression of c-ret during the development of identified spinal primary motoneurons. c-ret expression is regulated in a cell type-specific manner among the three primary motoneurons. c-ret is expressed at its highest levels in caudal primary (CaP) motoneurons and transcripts can be detected shortly before the expression of the CaP-specific gene, islet2. We suggest that c-ret may play a role in specifying CaP cell identity. c-ret is expressed at low levels in the other primary motoneurons and also in a subset of secondary motoneurons, suggesting that it may also play a broader role in motoneuronal survival or maintenance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping