PUBLICATION
Appendix: measurement of intracellular water volumne in multicompartmental systems such as the zebrafish embryo
- Authors
- Kleinhans, F.W. and Hagedorn, M.
- ID
- ZDB-PUB-970917-10
- Date
- 1997
- Source
- The Journal of experimental zoology 278(6): 368-371 (Journal)
- Registered Authors
- Hagedorn, Mary
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
Kleinhans, F.W. and Hagedorn, M. (1997) Appendix: measurement of intracellular water volumne in multicompartmental systems such as the zebrafish embryo. The Journal of experimental zoology. 278(6):368-371.
Abstract
Measurement of the total volume available todissolve a solute in biological systems is impor-tant for basic and applied studies in cryobiology,physiology, pharmacology, and other biomedicalfields. Electron Spin Resonance (ESR) permits ac-curate measurements of intracellular water vol-ume and has been applied to vesicles (Vistnes andPuskin, ’81), mammalian sperm (Hammerstedt etal., ’78; Kleinhans et al., ’92), and red blood cells(Moronne et al., ’90; Kleinhans at al., ’92). Thegoal of this appendix is to show in greater detailthe assumptions and modifications used in calcu-lating the intracellular water volume of thezebrafish embryo using ESR
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping