ZFIN ID: ZDB-PUB-961104-11
Bioconcentration, metabolism and toxicity of substituted anilines in the zebrafish (Brachydanio rerio)
Zok, S., Gorge, G., Kalsch, W., and Nagel, R.
Date: 1991
Source: The Science of the total environment   109-110: 411-21 (Journal)
Registered Authors: Nagel, Roland
Keywords: none
MeSH Terms:
  • Aniline Compounds/metabolism*
  • Aniline Compounds/pharmacokinetics
  • Aniline Compounds/toxicity
  • Animals
  • Biotransformation
  • Chromatography, High Pressure Liquid
  • Kinetics
  • Structure-Activity Relationship
  • Zebrafish/metabolism*
PubMed: 1815362 Full text @ Sci. Total Environ.
The LC50 (96 h) values and the bioconcentration factors (BCF) for nine anilines (aniline; 2-, 3-, 4-chloroaniline; 2-, 3-, 4-nitroaniline; 2,4- and 3,4-dichloroaniline) in the zebrafish (Brachydanio rerio) were determined. Biotransformation products of anilines in the zebrafish were analyzed by HPLC. The aim of the investigations was to find relationships between accumulation/elimination/metabolism and toxicity on the one hand and between chemical structure and biotransformation on the other. We found a good correlation of log BCF and of log Pow with log LC50. This concurs with the assumption that the internal dose determines the toxicological effect. All anilines investigated, with the exception of 2- and 4-nitroaniline, were transformed into the corresponding acetanilides. The toxicity of the compounds was not altered by biotransformation. A comparison of the extent of acetylation confirms the supposition that substituents in the ortho position sterically hinder acetylation.