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ZFIN ID: ZDB-PUB-961014-76
Expression of zebrafish nk2.2 is influenced by sonic hedgehog/vertebrate hedgehog-1 and demarcates a zone of neuronal differentiation in the embryonic forebrain
Barth, K.A. and Wilson, S.W.
Date: 1995
Source: Development (Cambridge, England)   121: 1755-1768 (Journal)
Registered Authors: Barth, Anukampa, Wilson, Steve
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/embryology*
  • Cell Differentiation
  • Eye/embryology
  • Gene Expression Regulation, Developmental*
  • Gene Transfer Techniques
  • Genes, Homeobox*
  • Hedgehog Proteins
  • Homeodomain Proteins/genetics*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Neurons/cytology
  • Neurons/physiology
  • Proteins/genetics*
  • Trans-Activators*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 7600991
We have isolated zebrafish nk2.2, a member of the Nk-2 family of homeobox genes. nk2.2 is expressed in a continuous narrow band of cells along a boundary zone demarcating the location at which two of the earliest nuclei in the brain differentiate. This band of cells is located within a few cell diameters of cells expressing the signalling molecule sonic hedgehog/vertebrate hedgehog-1 (shh/vhh-1). Injection of shh/vhh-1 RNA results in ectopic expression of nk2.2 and concomitant abnormalities in the forebrain and eyes. Moreover, cyclops mutant embryos, which initially lack neurectodermal expression of shh/vhh-1, show a concomitant lack of nk2.2 expression. Together, these results suggest a requirement of shh/vhh-1 protein for the spatial regulation of nk2.2 expression.