PUBLICATION
High velocity mechanical injection of foreign DNA into fish oocytes
- Authors
- Kolesnikov, V.A., Alimov, A.A., Barmintsev, V.A., Benyumov, A.O., Zelenina, I.A., Krasnov, A.M., Dzhabur, R., and Zelenin, A.V.
- ID
- ZDB-PUB-961014-619
- Date
- 1990
- Source
- Genetika 26(12): 2122-2126 (Journal)
- Registered Authors
- Benyumov, Alexey O.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cypriniformes/genetics*
- DNA/administration & dosage*
- Kanamycin Kinase
- Microinjections*
- Oocytes/metabolism
- Phosphotransferases/genetics
- Trout/genetics*
- Tungsten
- Zebrafish/genetics*
- beta-Galactosidase/genetics
- PubMed
- 1964924
Citation
Kolesnikov, V.A., Alimov, A.A., Barmintsev, V.A., Benyumov, A.O., Zelenina, I.A., Krasnov, A.M., Dzhabur, R., and Zelenin, A.V. (1990) High velocity mechanical injection of foreign DNA into fish oocytes. Genetika. 26(12):2122-2126.
Abstract
High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.
Errata / Notes
[Article in Russian]
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping