header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-961014-544
Zebrafish wnt8 and wnt8b share a common activity but are involved in distinct developmental pathways
Kelly, G.M., Greenstein, P., Erezyilmaz, D.F., and Moon, R.T.
Date: 1995
Source: Development (Cambridge, England) 121: 1787-1799 (Journal)
Registered Authors: Kelly, Greg, Moon, Randall T.
Keywords: Danio rerio, wnt, krox20, gsc, pax, snail1, spadetail, cyclops, mesoderm induction, pattern formation
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytoskeletal Proteins
  • DNA Primers
  • Embryonic Induction/genetics*
  • Female
  • Gene Expression
  • In Situ Hybridization
  • Mesoderm/physiology*
  • Molecular Sequence Data
  • Morphogenesis
  • Phenotype
  • Polymerase Chain Reaction
  • Proteins/genetics*
  • Sequence Alignment
  • Wnt Proteins
  • Xenopus
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins*
PubMed: 7600994
FIGURES
ABSTRACT
The specification of the vertebrate body plan is dependent on numerous signaling molecules, including members of the Wnt family. We have identified two zebrafish wnt8 paralogs related to Xwnt-8B and Xwnt-8, respectively. A RT-PCR assay demonstrated that wnt8 is expressed maternally, with transcripts detected throughout embryogenesis, whereas wnt8b transcripts were first detected during late gastrulation. The wnt8 transcripts at 50% epiboly are spatially restricted to those cells at the blastoderm margin, overlying gsc-expressing cells in the axial hypoblast. During late gastrulation, wnt8 was no longer detected in the marginal cells at the dorsal midline and by mid-segmentation, transcripts were found in the presumptive tail bud. In contrast, wnt8b expression is spatially restricted to prospective neuroepithelium, and later to neural-specific structures. Overexpression of both wnts results in two major phenotypes: radialized embryos and embryos with anterior defects. These phenotypes were preceded by significant changes in the spatial expression patterns of gsc and ntl transcripts, reminiscent of activities of Xwnt-8 in Xenopus, and consistent with a role for wnt8 in the specification or patterning of mesoderm.
ADDITIONAL INFORMATION