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ZIRC
ZFIN ID: ZDB-PUB-961014-378
Induction of recessive lethal mutations in zebrafish with ultraviolet light
Grunwald, D.J., and Streisinger, G.
Date: 1992
Source: Genetics research   59: 93-101 (Journal)
Registered Authors: Grunwald, David, Streisinger, George
Keywords: none
MeSH Terms:
  • Animals
  • DNA Damage
  • Female
  • Fertility/radiation effects
  • Genes, Lethal
  • Genotype
  • Male
  • Mosaicism
  • Mutagenesis
  • Mutagenesis, Site-Directed
  • Phenotype
  • Spermatozoa/radiation effects*
  • Ultraviolet Rays
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 1628821 Full text @ Genet. Res.
ABSTRACT
Recessive lethal germline and specific locus somatic mutations were induced efficiently in the zebrafish by exposure of mature sperm to UV light. Mutagenesis of sperm yielded mosaic individuals: clones bearing novel mutations represented approximately 12-25% of the haploid germ cells and 25-50% of the somatic tissue. Simple methods are described for the reliable identification and propagation of newly arising developmental mutations in zebrafish.
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