Grunwald, D.J., and Streisinger, G. (1992) Induction of recessive lethal mutations in zebrafish with ultraviolet light. Genetics research. 59:93-101.
Recessive lethal germline and specific locus somatic mutations were induced efficiently in the zebrafish by exposure of mature sperm to UV light. Mutagenesis of sperm yielded mosaic individuals: clones bearing novel mutations represented approximately 12-25% of the haploid germ cells and 25-50% of the somatic tissue. Simple methods are described for the reliable identification and propagation of newly arising developmental mutations in zebrafish.