PUBLICATION

The notochord and floor plate guide growth cones in the zebrafish spinal cord

Authors
Greenspoon, S., Patel, C.K., Hashmi, S., Bernhardt, R.R., and Kuwada, J.Y.
ID
ZDB-PUB-961014-373
Date
1995
Source
The Journal of neuroscience : the official journal of the Society for Neuroscience   15: 5956-5965 (Journal)
Registered Authors
Bernhardt, Robert, Kuwada, John
Keywords
none
MeSH Terms
  • Animals
  • Axons/physiology
  • Embryonic and Fetal Development
  • Neural Pathways/physiology
  • Notochord/physiology*
  • Spinal Cord/embryology*
  • Zebrafish/embryology*
PubMed
7666180
Abstract
Elimination of the floor plate, a row of distinctive cells at the ventral midline of the spinal cord, dramatically increased the frequency of errors made by specific growth cones in the zebrafish embryo. This demonstrated that the floor plate participated in guiding specific growth cones at the ventral midline of the spinal cord. However, since a significant proportion of these growth cones followed their normal pathway despite the absence of the floor plate, we hypothesized that a second source of cues must exist at or near the ventral midline. We tested whether the notochord, which is located just ventral to the spinal cord, was an additional source of pathfinding cues by eliminating it prior to axonogenesis. Laser ablation of the notochord in wildtype embryos increased errors by spinal growth cones. Likewise, spinal growth cones made errors in no tail mutants that are missing the notochord but not the floor plate. These results demonstrate that the notochord also participates in guiding growth cones at the ventral midline. Furthermore, removal of both the floor plate and notochord resulted in a higher error rate than removal of either one alone. These results suggest that the notochord and floor plate normally act in concert to guide spinal growth cones at the ventral midline.
Genes / Markers
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
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Errata and Notes