PUBLICATION
Genetic heterogeneity in isogenic homozygous clonal zebrafish
- Authors
- Buth, D.G., Gordon, M.S., Plaut, I., Drill, S.L., and Adams, L.G.
- ID
- ZDB-PUB-961014-150
- Date
- 1995
- Source
- Proceedings of the National Academy of Sciences of the United States of America 92: 12367-12369 (Journal)
- Registered Authors
- Gordon, Malcolm S., Plaut, Itai
- Keywords
- none
- MeSH Terms
-
- Alleles
- Animals
- Chromosome Mapping*
- Diploidy
- Enzymes/genetics*
- Female
- Genetic Variation*
- Genotype
- Glucose-6-Phosphate Isomerase/biosynthesis
- Glucose-6-Phosphate Isomerase/genetics
- Homozygote*
- Isoenzymes/genetics
- Malate Dehydrogenase/biosynthesis
- Malate Dehydrogenase/genetics*
- Male
- Muscle, Skeletal/enzymology
- Mutation*
- Zebrafish/genetics*
- PubMed
- 8618902 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Buth, D.G., Gordon, M.S., Plaut, I., Drill, S.L., and Adams, L.G. (1995) Genetic heterogeneity in isogenic homozygous clonal zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 92:12367-12369.
Abstract
The C32 isogenic homozygous diploid (IHD) strain of the zebrafish (Danio rerio) was found to be polyallelic at a malate dehydrogenase locus (sMdh-A). A variant allele is thought to have arisen via mutation within the past 10 bisexual generations that have maintained the strain since its last gynogenetic cloning event; this unique allele now predominates at the sMdh-A locus. The estimated mutation rate in this species is sufficiently high that long-term genetic homogeneity of its IHD clones cannot be assumed. Researchers using such bisexually maintained clones should be aware that they are not necessarily using genetically uniform subjects. Genetic uniformity of cloned IHD zebrafish will be maximized if experimental subjects are obtained soon after a cloning event.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping