PUBLICATION
Detection of neuron-specific protein gene product (PGP) 9.5 in the rat and zebrafish using anti-human PGP9.5 antibodies
- Authors
- Trowern, A.R., Laight, R., Maclean, N., and Mann, D.A.
- ID
- ZDB-PUB-961014-1141
- Date
- 1996
- Source
- Neuroscience letters 210: 21-24 (Journal)
- Registered Authors
- Maclean, Norman
- Keywords
- none
- MeSH Terms
-
- Animals
- Antibodies, Monoclonal*
- Antibody Specificity
- Blotting, Western
- Central Nervous System/chemistry
- Enzyme-Linked Immunosorbent Assay
- Eye/chemistry
- Gills/chemistry
- Humans
- Nerve Tissue Proteins/analysis*
- Nerve Tissue Proteins/immunology*
- PC12 Cells/chemistry
- Rats
- Skin/chemistry
- Thiolester Hydrolases/analysis*
- Thiolester Hydrolases/immunology*
- Ubiquitin Thiolesterase
- Viscera/chemistry
- Zebrafish
- PubMed
- 8762182 Full text @ Neurosci. Lett.
Citation
Trowern, A.R., Laight, R., Maclean, N., and Mann, D.A. (1996) Detection of neuron-specific protein gene product (PGP) 9.5 in the rat and zebrafish using anti-human PGP9.5 antibodies. Neuroscience letters. 210:21-24.
Abstract
Protein gene product (PGP) 9.5 is a developmentally regulated neuron- and neuroendocrine cell-specific ubiquitin carboxy- terminal hydrolase (UCHL1) expressed throughout the mammalian central and peripheral nervous systems. We have compared the use of monoclonal and polyclonal antibodies raised against human PGP9.5 for immunodetection of the protein in tissues of the zebrafish and rat. We show that a monoclonal antibody 13C4, which recognises an N-terminal epitope, detects PGP9.5 on Western blots as a single 27 kDa band present at high levels in zebrafish and rat brain. By contrast, the polyclonal antisera recognises multiple tissue-specific proteins in the rat and fails to detect PGP9.5 in the zebrafish. Finally, we have developed a specific ELISA assay for detection of cellular PGP9.5 using MAb13C4 and have employed the assay to show that PGP9.5 is not upregulated during nerve growth factor (NGF)-induced differentiation of rat PC12 cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping