PUBLICATION
Real-Time Observation of Germinal Vesicle Migration During Oocyte Meiotic Cell Division Using Ovarian Fluorescent Transgenic Zebrafish
- Authors
- Tsutsumi, E., Tokumoto, T.
- ID
- ZDB-PUB-240416-22
- Date
- 2024
- Source
- Zebrafish 21: 171176171-176 (Journal)
- Registered Authors
- Tokumoto, Toshinobu
- Keywords
- GFP, germinal vesicle, oocyte maturation
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Division
- Ectoderm/embryology*
- Embryonic Structures*
- Female
- Oocytes
- Ovary*
- Zebrafish*
- PubMed
- 38621215 Full text @ Zebrafish
Citation
Tsutsumi, E., Tokumoto, T. (2024) Real-Time Observation of Germinal Vesicle Migration During Oocyte Meiotic Cell Division Using Ovarian Fluorescent Transgenic Zebrafish. Zebrafish. 21:171176171-176.
Abstract
The transgenic (TG) zebrafish allows researchers to bio-image specific biological phenomena in cells and tissues in vivo. We established TG lines to monitor changes in the ovaries of live fish. The original TG line with ovarian fluorescence was occasionally established. Although the cDNA integrated into the line was constructed for the expression of enhanced green fluorescent protein (EGFP) driven by the medaka β-actin promoter, the expression of EGFP is restricted to the oocytes and gills in adult fish. Furthermore, we found that germinal vesicles (GVs) in oocytes of the established line can be observed by relatively strong fluorescence around the GV. In this study, we tried to capture the dynamic processes of germinal vesicle breakdown (GVBD) during meiotic cell division using the GV fluorescent oocytes. As a result, GV migration and GVBD could be monitored in real time. We also succeeded in observing actin filaments involved in the migration of GV to the animal pole. This strain can be used for education in the process of oocyte meiotic cell division.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping