PUBLICATION
Laboratory Course Using Zebrafish to Uncover Changing Roles of Wnt Signaling in Early Vertebrate Development
- Authors
- Fiegl, M., Kimmel, R.A.
- ID
- ZDB-PUB-240416-19
- Date
- 2024
- Source
- Zebrafish 21: 128136128-136 (Journal)
- Registered Authors
- Kimmel, Robin
- Keywords
- Wnt signaling, axis specification, embryogenesis, patterning, zebrafish
- MeSH Terms
-
- Animals
- Body Patterning
- Embryo, Nonmammalian/metabolism
- Embryonic Development
- Gene Expression Regulation, Developmental
- Humans
- Wnt Signaling Pathway*
- Zebrafish*/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38621212 Full text @ Zebrafish
Citation
Fiegl, M., Kimmel, R.A. (2024) Laboratory Course Using Zebrafish to Uncover Changing Roles of Wnt Signaling in Early Vertebrate Development. Zebrafish. 21:128136128-136.
Abstract
Coordinated signaling pathway activity directs early patterning to set up the vertebrate body plan. Perturbations in the timing or location of signal molecule expression impacts embryo morphology and organ formation. In this study, we present a laboratory course to use zebrafish for studying the role of Wnt signaling in specifying the early embryonic axes. Students are exposed to basic techniques in molecular and developmental biology, including embryo manipulation, fluorescence microscopy, image processing, and data analysis. Furthermore, this course incorporates student-designed experiments to stimulate independent inquiry and improve scientific learning, providing an experience resembling graduate-level laboratory research. Students appreciated following vertebrate development in real-time, and principles of embryogenesis were reinforced by observing the morphological changes that arise due to signaling alterations. Scientific and research skills were enhanced through practice in experimental design, interpretation, and presentation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping