PUBLICATION
Long-term exposure to aryl hydrocarbon receptor agonist neburon induces reproductive toxicity in male zebrafish (Danio rerio)
- Authors
- Zhang, F., Tang, C., Zhu, Y., Wang, Q., Huang, X., Yang, C., He, C., Zuo, Z.
- ID
- ZDB-PUB-240326-7
- Date
- 2024
- Source
- Journal of environmental sciences (China) 142: 193203193-203 (Journal)
- Registered Authors
- He, Chengyong
- Keywords
- AhR agonist, HPG axis, Male reproductive toxicity, Neburon, Zebrafish
- MeSH Terms
-
- Animals
- Male
- Phenylurea Compounds/pharmacology
- Receptors, Aryl Hydrocarbon/metabolism
- Reproduction
- Water Pollutants, Chemical*/metabolism
- Zebrafish*/metabolism
- PubMed
- 38527884 Full text @ J. Environ. Sci. (China).
Citation
Zhang, F., Tang, C., Zhu, Y., Wang, Q., Huang, X., Yang, C., He, C., Zuo, Z. (2024) Long-term exposure to aryl hydrocarbon receptor agonist neburon induces reproductive toxicity in male zebrafish (Danio rerio). Journal of environmental sciences (China). 142:193203193-203.
Abstract
Neburon is a phenylurea herbicide that is widely used worldwide, but its toxicity is poorly studied. In our previous study, we found that neburon has strong aryl hydrocarbon receptor (AhR) agonist activity, but whether it causes reproductive toxicity is not clear. In the present study, zebrafish were conducted as a model organism to evaluate whether environmental concentrations of neburon (0.1, 1 and 10 µg/L) induce reproductive disorder in males. After exposure to neburon for 150 days from embryo to adult, that the average spawning egg number in high concentration group was 106.40, which was significantly lower than 193.00 in control group. This result was mainly due to the abnormal male reproductive behavior caused by abnormal transcription of genes associated with reproductive behavior in the brain, such as secretogranin-2a. The proportions of spermatozoa in the medium and high concentration groups were 82.40% and 83.84%, respectively, which were significantly lower than 89.45% in control group. This result was mainly caused by hormonal disturbances and an increased proportion of apoptotic cells. The hormonal disruption was due to the significant changes in the transcription levels of key genes in the hypothalamus-pituitary-gonadal axis following neburon treatment. Neburon treatment also significantly activated the AhR signaling pathway, causing oxidative stress damage and eventually leading to a significant increase in apoptosis in the exposed group. Together, these data filled the currently more vacant profile of neburon toxicity and might provide information to assess the ecotoxicity of neburon on male reproduction at environmentally relevant concentrations.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping