PUBLICATION
Assessments of carbon nanotubes toxicities in zebrafish larvae using multiple physiological and molecular endpoints
- Authors
- Audira, G., Lee, J.S., Vasquez, R.D., Roldan, M.J.M., Lai, Y.H., Hsiao, C.D.
- ID
- ZDB-PUB-240308-8
- Date
- 2024
- Source
- Chemico-biological interactions 392: 110925 (Journal)
- Registered Authors
- Hsiao, Chung-Der
- Keywords
- Carbon nanotubes, Embryo, Larva, Toxicity, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Larva
- Nanotubes, Carbon*/toxicity
- Oxidative Stress
- Reactive Oxygen Species/metabolism
- Water Pollutants, Chemical*/toxicity
- Zebrafish/metabolism
- PubMed
- 38452846 Full text @ Chem. Biol. Interact.
Citation
Audira, G., Lee, J.S., Vasquez, R.D., Roldan, M.J.M., Lai, Y.H., Hsiao, C.D. (2024) Assessments of carbon nanotubes toxicities in zebrafish larvae using multiple physiological and molecular endpoints. Chemico-biological interactions. 392:110925.
Abstract
In recent years, carbon nanotubes (CNTs) have become one of the most promising materials for the technology industry. However, due to the extensive usage of these materials, they may be released into the environment, and cause toxicities to the organism. Here, their acute toxicities in zebrafish embryos and larvae were evaluated by using various assessments that may provide us with a novel perspective on their effects on aquatic animals. Before conducting the toxicity assessments, the CNTs were characterized as multiwall carbon nanotubes (MWCNTs) functionalized with hydroxyl and carboxyl groups, which improved their solubility and dispersibility. Based on the results, abnormalities in zebrafish behaviors were observed in the exposed groups, indicated by a reduction in tail coiling frequency and alterations in the locomotion as the response toward photo and vibration stimuli that might be due to the disruption in the neuromodulatory system and the formation of reactive oxygen species (ROS) by MWCNTs. Next, based on the respiratory rate assay, exposed larvae consumed more oxygen, which may be due to the injuries in the larval gill by the MWCNTs. Finally, even though no irregularity was observed in the exposed larval cardiac rhythm, abnormalities were shown in their cardiac physiology and blood flow with significant downregulation in several cardiac development-related gene expressions. To sum up, although the following studies are necessary to understand the exact mechanism of their toxicity, the current study demonstrated the environmental implications of MWCNTs in particularly low concentrations and short-term exposure, especially to aquatic organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping