PUBLICATION
Dynamics of Chromosome Aberrations and Cell Death in Zebrafish Embryos Exposed to 137Cs Total-Body Irradiation
- Authors
- Asana Marican, H.T., Shen, H.
- ID
- ZDB-PUB-240125-8
- Date
- 2024
- Source
- Environmental science & technology 58(5): 2204-2213 (Journal)
- Registered Authors
- Shen, Hongyuan
- Keywords
- aneuploidy, apoptosis, chromatin, cytogenetics, genotoxicity, progenitors, radiation, radiosensitivity
- MeSH Terms
-
- Animals
- Apoptosis
- Chromosome Aberrations*
- Chromosomes
- Embryo, Nonmammalian/radiation effects
- Gamma Rays
- Zebrafish*/genetics
- PubMed
- 38269402 Full text @ Env. Sci. Tech.
Citation
Asana Marican, H.T., Shen, H. (2024) Dynamics of Chromosome Aberrations and Cell Death in Zebrafish Embryos Exposed to 137Cs Total-Body Irradiation. Environmental science & technology. 58(5):2204-2213.
Abstract
Ionizing radiation exposure induces significant DNA damage and cell death in aquatic species. Accurate sensing and quantification play pivotal roles in environmental monitoring and surveillance. Zebrafish (Danio rerio) is a well-suited animal model for research into this aspect, especially with recent development of cytogenetic and transgenic tools. In this study, we present time-course studies of chromosome aberrations and cell death in zebrafish embryos exposed to 2 Gy 137Cs total-body irradiation. Using a cytogenetic approach, we quantified chromosome and chromatid aberrations in irradiated embryos at 6, 14, 20, and 24 h postirradiation. Metaphases with aberrations showed rapid declining kinetics, accompanied by incomplete karyotypes and irregular chromatin contents. Using an apoptosis-reporting transgenic zebrafish, we found increasing cell death along these time points, with the embryonic eyes and brain contributing the majority of the cell death volumes. We provide evidence that self-proliferating progenitor cells form the underlying linkage between the two kinetics and their positions define radiosensitive niches in zebrafish embryos. Our results provide detailed chromosome aberration and cell death dynamics in 137Cs-irradiated zebrafish embryos and unveil the appropriate timeline and tissue positions for accurate sensing and quantification of radiation-induced damages in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping