PUBLICATION
All-optical interrogation of brain-wide activity in freely swimming larval zebrafish
- Authors
- Chai, Y., Qi, K., Wu, Y., Li, D., Tan, G., Guo, Y., Chu, J., Mu, Y., Shen, C., Wen, Q.
- ID
- ZDB-PUB-240111-24
- Date
- 2023
- Source
- iScience 27: 108385108385 (Journal)
- Registered Authors
- Wen, Quan
- Keywords
- Behavioral neuroscience, Biocomputational method, Neuroscience
- MeSH Terms
- none
- PubMed
- 38205255 Full text @ iScience
Citation
Chai, Y., Qi, K., Wu, Y., Li, D., Tan, G., Guo, Y., Chu, J., Mu, Y., Shen, C., Wen, Q. (2023) All-optical interrogation of brain-wide activity in freely swimming larval zebrafish. iScience. 27:108385108385.
Abstract
We introduce an all-optical technique that enables volumetric imaging of brain-wide calcium activity and targeted optogenetic stimulation of specific brain regions in unrestrained larval zebrafish. The system consists of three main components: a 3D tracking module, a dual-color fluorescence imaging module, and a real-time activity manipulation module. Our approach uses a sensitive genetically encoded calcium indicator in combination with a long Stokes shift red fluorescence protein as a reference channel, allowing the extraction of Ca2+ activity from signals contaminated by motion artifacts. The method also incorporates rapid 3D image reconstruction and registration, facilitating real-time selective optogenetic stimulation of different regions of the brain. By demonstrating that selective light activation of the midbrain regions in larval zebrafish could reliably trigger biased turning behavior and changes of brain-wide neural activity, we present a valuable tool for investigating the causal relationship between distributed neural circuit dynamics and naturalistic behavior.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping