PUBLICATION
Live imaging of cutaneous wound healing after rotary tool injury in zebrafish
- Authors
- Greenspan, L.J., Ameyaw, K.K., Castranova, D., Mertus, C.A., Weinstein, B.M.
- ID
- ZDB-PUB-231120-10
- Date
- 2023
- Source
- The Journal of investigative dermatology 144(4): 888-897.e6 (Journal)
- Registered Authors
- Ameyaw, Keith, Castranova, Dan, Greenspan, Leah, Weinstein, Brant M.
- Keywords
- cutaneous wound healing, imaging, zebrafish
- MeSH Terms
-
- Adult
- Aged
- Animals
- Humans
- Inflammation
- Re-Epithelialization
- Skin*/diagnostic imaging
- Skin*/injuries
- Wound Healing/physiology
- Zebrafish*
- PubMed
- 37979772 Full text @ J. Invest. Dermatol.
Citation
Greenspan, L.J., Ameyaw, K.K., Castranova, D., Mertus, C.A., Weinstein, B.M. (2023) Live imaging of cutaneous wound healing after rotary tool injury in zebrafish. The Journal of investigative dermatology. 144(4):888-897.e6.
Abstract
Cutaneous wounds are common afflictions that follow a stereotypical healing process involving hemostasis, inflammation, proliferation, and remodeling phases. In the elderly and those suffering from vascular or metabolic diseases, poor healing following cutaneous injuries can lead to open chronic wounds susceptible to infection. The discovery of new therapeutic strategies to improve this defective wound healing requires a better understanding of the cellular behaviors and molecular mechanisms that drive the different phases of wound healing and how these are altered with age or disease. The zebrafish provides an ideal model for visualization and experimental manipulation of the cellular and molecular events during wound healing in the context of an intact, living vertebrate. To facilitate studies of cutaneous wound healing in the zebrafish, we have developed an inexpensive, simple, and effective method for generating reproducible cutaneous injuries in adult zebrafish using a rotary tool. We demonstrate that our injury system can be used in combination with high-resolution live imaging to monitor skin re-epithelialization, immune cell recruitment and activation, and vessel regrowth in the same animal over time. This injury system provides a valuable experimental platform to study key cellular and molecular events during wound healing in vivo with unprecedented resolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping