PUBLICATION
Kidney injury contributes to edema of zebrafish larvae caused by quantum dots
- Authors
- Wang, L., Liang, C., Zheng, N., Yang, C., Yan, S., Wang, X., Zuo, Z., He, C.
- ID
- ZDB-PUB-231115-5
- Date
- 2023
- Source
- The Science of the total environment 908: 168420 (Journal)
- Registered Authors
- He, Chengyong
- Keywords
- Edema, Fish, Nanoparticle, Osmoregulation, Pronephros injury
- MeSH Terms
-
- Animals
- Kidney Glomerulus
- Larva
- Quantum Dots*/chemistry
- Quantum Dots*/toxicity
- Zebrafish*/genetics
- PubMed
- 37963533 Full text @ Sci. Total Environ.
Citation
Wang, L., Liang, C., Zheng, N., Yang, C., Yan, S., Wang, X., Zuo, Z., He, C. (2023) Kidney injury contributes to edema of zebrafish larvae caused by quantum dots. The Science of the total environment. 908:168420.
Abstract
Edema represents a notable outcome in fishes exposed to aquatic pollutants, yet the underlying etiology remains inadequately understood. This investigation delves into the etiological factors of edema formation in 7 days post fertilization (dpf) zebrafish larvae following their exposure to InP/ZnS quantum dots (QDs), which was chosen as a prototypical edema inducer. Given the fundamental role of the kidney in osmoregulation, we used transgenic zebrafish lines featuring fluorescent protein labeling of the glomerulus, renal tubule, and blood vessels, in conjunction with histopathological scrutiny. We identified the pronounced morphological and structural aberrations within the pronephros. By means of tissue mass spectrometry imaging and hyperspectral microscopy, we discerned the accumulation of InP/ZnS QDs in the pronephros. Moreover, InP/ZnS QDs impeded the renal clearance capacity of the pronephros, as substantiated by diminished uptake of FITC-dextran. InP/ZnS QDs also disturbed the expression levels of marker genes associated with kidney development and osmoregulatory function at the earlier time points, which preceded the onset of edema. These results suggest that impaired fluid clearance most likely resulting from pronephros injury contributes to the emergence of zebrafish edema. Briefly, our study provides a perspective: the kidney developmental injury induced by exogenous substances may regulate edema in a zebrafish model.
Errata / Notes
This article is corrected by ZDB-PUB-231209-7.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping