PUBLICATION
Panoramic Dental X-ray Exposure Leads to Oxidative stress, Inflammation and Apoptosis-mediated Developmental Defects in Zebrafish Embryos
- Authors
- Karagöz, A., Beler, M., Altun, B.D., Ünal, İ., Cansız, D., Gündüz, H., Alturfan, A.A., Emekli-Alturfan, E., Erçalık-Yalçınkaya, Ş.
- ID
- ZDB-PUB-231023-55
- Date
- 2023
- Source
- Journal of stomatology, oral and maxillofacial surgery 124(6S): 101661 (Journal)
- Registered Authors
- Beler, Merih, Emekli-Alturfan, Ebru
- Keywords
- Diagnostic X-rays, apoptosis, development, panoramic, zebrafish embryos
- MeSH Terms
-
- Animals
- Apoptosis/genetics
- Embryo, Nonmammalian*/metabolism
- Humans
- Inflammation/chemically induced
- Inflammation/metabolism
- Oxidative Stress/genetics
- X-Rays
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 37866507 Full text @ J Stomatol Oral Maxillofac Surg
Citation
Karagöz, A., Beler, M., Altun, B.D., Ünal, İ., Cansız, D., Gündüz, H., Alturfan, A.A., Emekli-Alturfan, E., Erçalık-Yalçınkaya, Ş. (2023) Panoramic Dental X-ray Exposure Leads to Oxidative stress, Inflammation and Apoptosis-mediated Developmental Defects in Zebrafish Embryos. Journal of stomatology, oral and maxillofacial surgery. 124(6S):101661.
Abstract
Panoramic x-ray units are widely used in dental radiodiagnostics. Patients are exposed to relatively low radiation doses with panoramic imaging, but considering lifetime frequency of exposure, even a small risk can have serious health consequences. Our aim was to assess the effects of panoramic x-rays at two different exposure times on developing zebrafish embryos, focusing on oxidative stress, inflammation, apoptotic pathways, and development. Zebrafish embryos were divided into three groups: control, standard panoramic (SPE, 5.5 seconds exposure time) and pedodontic panoramic x-ray group (PPE, 4.8 seconds). Optically stimulated luminescence dosimeters were used to measure absorbed doses. Mean radiation doses for SPE and PPE were 7.83 mSv and 5.83 mSv respectively. At the end of 96 hours post-fertilization, lipid peroxidation (LPO), nitric oxide (NO), reduced glutathione (GSH), glutathione S-transferase and superoxide dismutase were measured in the embryos. Expressions of genes related with inflammation (tnfα, il6, ill15, il21), immunoregulation (ifng) and apoptosis (p53, bax, casp2, casp3, casp8) were determined by RT-PCR. Even at reduced doses at high-speed mode, developmental toxicity was observed in both groups as evidenced by decreased pigmentation, yolk sac oedema, and spinal curvature. While deterioration of oxidant-antioxidant balance, suppression of immune response, induction of inflammation and apoptosis were observed through increased LPO, NO, decreased GSH, ifng, and increased expressions of genes related with inflammation and apoptosis, these effects were more pronounced in the SPE group. These results demonstrate the influence of exposure time and indicate the need for further consideration of optimal panoramic modes from a radiation-induced damage perspective.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping